SPIO标记对成骨样分化细胞IRP1基因及蛋白表达的影响

发布时间：2018-01-17 16:23

本文关键词：SPIO标记对成骨样分化细胞IRP1基因及蛋白表达的影响　出处：《中山大学学报(医学科学版)》2013年02期 　论文类型：期刊论文

【摘要】：【目的】探讨超顺磁性氧化铁(SPIO)标记大鼠脂肪间充质干细胞(RADSC)后,其成骨样分化细胞中铁调节蛋白1(IRP1)基因及蛋白表达的变化。【方法】本实验采用转染剂多聚赖氨酸(PLL)介导SPIO标记RADSC,然后将其诱导为成骨样分化细胞,分别在诱导后0、7、14、21 d进行RT-PCR及Western blot检测其IRP1基因及蛋白的表达。【结果】成骨样分化细胞IRP1 mRNA表达水平在SPIO标记与未标记组之间差异均无统计学显著性意义(F=1.571,P=0.239);IRP1蛋白表达水平在标记与未标记组之间差异均无统计学显著性意义(F=0.547,P=0.500)。【结论】SPIO标记并未对成骨样分化细胞的IRP1基因及IRP1蛋白表达产生明显影响。
[Abstract]:[objective] to investigate the labeling of rat adipose mesenchymal stem cells (RADSCs) by superparamagnetic iron oxide (SPIO). The changes of iron regulatory protein 1 (IRP1) gene and protein expression in osteoblast-like differentiation cells. [methods] SPIO labeled RADSC was mediated by polylysine transducer (PLL). Then they were induced into osteoblast-like differentiation cells, respectively. RT-PCR and Western blot were used to detect the expression of IRP1 gene and protein in 21 days. [results] Osteoblast-like differentiation cell IRP1. There was no significant difference in the expression of mRNA between SPIO labeled and unlabeled groups (P < 0.05). FN 1.571. Pu 0.239; There was no significant difference in the expression of IRP1 protein between labeled and unlabeled groups. [conclusion] SPIO labeling has no significant effect on the expression of IRP1 gene and IRP1 protein in osteoblast-like differentiation cells.
【作者单位】：南方医科大学;广东省人民医院;广东省医学科学院放射科;
【基金】：国家自然科学基金(30970798) 广东省医学科研基金(A2011036,B2011017)
【分类号】：R329
【正文快照】： 干细胞移植在修复损伤组织如骨骼组织方面具有非常光明及诱人的前景[1]。但干细胞移植入活体动物后的示踪技术一直是制约干细胞研究发展的瓶颈环节。使用(superparamagnetic iron oxide,SPIO)标记干细胞,然后利用核磁共振成像(magnetic resonance imaging,MRI)无创地追踪干细

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