IL-17等炎性因子在风湿免疫相关性血细胞减少骨髓细胞中的分泌状态及其临床意义

发布时间：2018-01-18 13:33

  本文关键词：IL-17等炎性因子在风湿免疫相关性血细胞减少骨髓细胞中的分泌状态及其临床意义　出处：《中国免疫学杂志》2013年01期 　论文类型：期刊论文

  更多相关文章： 风湿免疫相关性血细胞减少 嗜酸性粒细胞 IL- IL- 炎性因子 造血微环境 复方丹参

【摘要】：目的:观察风湿免疫相关性血细胞减少(Rheumatism immune-related hematocytopenia,RIRH)患者骨髓免疫细胞炎性因子分泌状态,探讨其对骨髓造血细胞损伤的病理作用及临床意义。方法:①ELISA法检测66例RIRH患者血清IL-5、IL-10、IL-12、IL-17和IFN-γ浓度,对照组30例采自健康查体血清;免疫化学法观察骨髓免疫细胞HLA-DR表达;②免疫荧光法观察骨髓细胞抗人球蛋白(IgG)抗体、FcγⅡ受体(FcγⅡR)、甘露糖受体(MR)、ICAM-1、IL-12、IL-17A与IL-17RA表达;30例缺铁性贫血患者骨髓作为疾病对照;③随机抽取32例血小板50×109L-1无出血倾向患者,常规治疗同时联合复方丹参促进炎性因子代谢,与30例不联合复方丹参患者对照治疗效果。结果:①66例患者血清IL-5、IL-10、IL-12、IL-17和IFN-γ浓度分别为(126.04±7.66)ng/L,(316.23±25.163)ng/L,(328.43±27.01)ng/L,(48.17±4.22)ng/L和(40.28±3.69)ng/L,较之对照组(33.16±2.17)ng/L,(176.25±10.69)ng/L,(94.05±3.26)ng/L,(20.03±1.14)ng/L和(8.25±2.01)ng/L显著升高;②骨髓中活化的组织嗜碱细胞、嗜酸性粒细胞(EOS)、树突状细胞和巨噬细胞(Mφ)等免疫细胞高表达HLA-DR、FcγⅡR、MR、ICAM-1、IL-12、IL-17A和IL-17RA;③32例IRHS患者联合复方丹参组经治疗2周后,血清IL-5和IL-17含量较之对照组显著降低,血象恢复时间较之对照组提前1周。结论:RIRH患者骨髓中多种免疫细胞活化,表达和分泌多种免疫分子,介导ADCC效应、细胞免疫功能和炎性反应,是损伤病变的造血细胞的重要免疫机制。抗感染、抑制免疫联合复方丹参活血化瘀,能改善骨髓造血微环境,促进造血恢复。
[Abstract]:Objective: To observe the immune related hematocytopenia rheumatism (Rheumatism immune-related, hematocytopenia, RIRH) in patients with bone marrow cells secretion of inflammatory factors, and explore the pathological role of bone marrow hematopoietic cell injury and its clinical significance. Methods: detection of 66 cases by ELISA RIRH in sera of patients with IL-5, IL-10, IL-12, IL-17 concentration and IFN- gamma, control group of 30 cases collected from healthy serum; immunohistochemical method was used to observe the expression of HLA-DR in bone marrow cells; bone marrow cells were observed by immunofluorescence anti human globulin (IgG) antibody, Fc receptor gamma (Fc gamma R), mannose receptor (MR), ICAM-1, IL-12, IL-17RA and IL-17A expression in 30 cases; iron deficiency anemia in patients with bone marrow as a disease control; the randomly selected 32 cases of platelet 50 * 109L-1 patients with bleeding tendency, and routine treatment combined with compound salvia miltiorrhiza promotes inflammatory factor and metabolism, 30 cases of patients treated with Compound Danshen control 鐤楁晥鏋，

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