重组人松弛素对舒张性心力衰竭模型兔心脏功能的影响研究
发布时间：2018-01-18 14:26
本文关键词：重组人松弛素对舒张性心力衰竭模型兔心脏功能的影响研究 出处：《安徽医科大学》2017年硕士论文 论文类型：学位论文
更多相关文章： 重组人松弛素 心力衰竭 舒张性 心脏超声 心室重塑
【摘要】：【目的】研究重组人松弛素(recombinant human relaxin,rh-RLX)对舒张性心力衰竭(Distolic Heart Failure,DHF)模型兔心脏功能的影响。【方法】1.选取36只成年新西兰家兔,按数字表法随机分为手术组(n=30只)和假手术组(n=6),采用腹主动脉缩窄术(缩窄50%-60%)建立DHF动物模型,假手术组仅开腹游离出腹主动脉但不缩窄。术后第8周行动物心脏超声心动图检查,根据2007年欧洲心脏病学会及超声协会推出的DHF诊断标准:左心室射血分数(LVEF)㧐50%且二尖瓣舒张早期血流峰值/舒张晚期血流峰值(E/A㩳1)为DHF建模成功标准。2.将建模成功的手术组家兔随机分为DHF组(n=6)、洛丁新组(n=8,给药剂量:3mg/(kg·d),)、小剂量组(n=8,给药剂量:rh-RLX 30ug/(kg·d))、大剂量组(n=8,给药剂量:rh-RLX 98ug/(kg·d)),DHF组与假手术组正常饲养作空白对照组,各组连续给药1周。3.所有分组家兔于术前、术后第8周、术后第9周三个时间点行心脏超声心动图检查,通过成组资料T检验与配对样本T检验比较各组家兔心脏功能变化情况。4.术后第9周,将上述5组家兔分别在行心脏超声心动图检查后,空气栓塞法处死各组家兔,分别留取左心室心肌在我院病理科制作家兔心肌病理切片并采用Masson染色法观察各组家兔心肌间质纤维增生变化情况。【结果】1.心脏超声心动图结果:(1).术后第8周,与假手术组相比,手术组家兔心脏收缩功能指标左心室射血分数(LVEF)(76.97±3.61vs75.90±1.67,p㧐0.05)正常,差异无统计学意义,而各舒张功能指标具有统计学差异,表现为左心室后壁厚度(LVPW)(2.48±0.25mm vs 4.86±0.17mm,p㩳0.01)、室间隔厚度(IVS)(2.72±0.31mm vs3.20±0.10mm,p㩳0.01)明显增厚、E/A比值(1.55±0.31vs0.84±0.07,p㩳0.01)明显下降、左心室舒张末压(LVEDP)(4.09±0.42mm Hg vs 11.7±1.26mm Hg,p㩳0.01)显著升高;(2)术后第9周,与DHF组比较,大剂量组E/A显著增加(1.36±0.10 vs 0.83±0.06,p㩳0.01),LVPW(3.71±0.21mm vs 4.87±0.08mm,p㩳0.01)、IVS(3.10±0.08mm vs3.23±0.08mm,p㩳0.05)、LVEDP(8.74±1.16 mm Hg vs 12.07±1.31mm Hg,p㩳0.01)均显著降低。而与DHF组比较,洛丁新组和小剂量组各心功能指标均无显著差异(p㧐0.05);(3)术后第9周,与洛丁新组比较,大剂量组E/A(1.36±0.10 vs 0.87±0.05,p㩳0.05)显著增加、LVPW(3.71±0.21mm vs 4.75±0.18mm,p㩳0.01)及LVEDP(8.74±1.16mm Hg vs 12.07±1.31 mm Hg,p㩳0.01)均显著下降,余心功能指标均无显著差异性(p㧐0.05),而与洛丁新组比较,小剂量组各心功能指标均无显著差异性(p㧐0.05)。2.心肌Masson染色结果显示:洛丁新组、rh-RLX小剂量组、rh-RLX大剂量组心肌间质纤维增生状况较DHF组均有不同程度减轻,其中改善最显著的是rh-RLX大剂量组,主要表现在该组增生的心肌间质纤维显著减少仅残存少许纤维条,心肌细胞内浸润的慢性炎症细胞完全消失,心肌细胞体积及胞质形态均完全恢复正常。【结论】本研究结果证实采用大剂量rh-RLX治疗DHF模型兔,可明显减轻DHF兔心肌纤维化程度从而改善心脏舒张功能,其机制可能与rh-RLX具有抗心肌纤维化功能密切相关。
[Abstract]:[Objective] to study the effects of recombinant human relaxin (recombinant human, relaxin, rh-RLX) on diastolic heart failure (Distolic Heart Failure, DHF) on cardiac function in rabbit model. [Methods] 1. select 36 adult New Zealand rabbits were randomly divided into operation group (n= = 30) and sham operation group (n=6), coarctation of the abdominal aortic (narrow 50%-60%) to establish an animal model of DHF, the sham operation group only open free abdominal aortic coarctation. But not after eighth weeks for animal echocardiography, according to the diagnostic criteria of DHF in 2007 the European Society of Cardiology and ultrasound Association launched: left ventricular ejection fraction (LVEF)? 50% and mitral early diastolic peak / late diastolic peak flow (E/A? 1) DHF modeling success criteria.2. will modeling successful surgical group rabbits were randomly divided into DHF group (n=6), Lotensin group (n=8 dosage: 3mg/ (kg, d), small) dose group ( N=8 dose: rh-RLX 30ug/ (kg - D), high dose group (n=8), dose: rh-RLX 98ug/ (kg, d)), DHF group and sham operation group were normal control group, each group for 1 weeks in all groups of rabbits in.3. before surgery, eighth weeks postoperatively after Wednesday, after ninth time points for echocardiography by grouped data T test and paired samples T test were compared ninth weeks of rabbit cardiac function changes after.4., the 5 groups of rabbits were in echocardiography, air embolism death rabbits were collected, left ventricular in pathology department rabbit myocardial pathology and the Masson were observed in rabbits of myocardial interstitial fibrosis changes stain method. [results] 1. echocardiography results: (1). After eighth weeks, compared with sham operation group, operation group of rabbit cardiac systolic function indexes of left 蹇冨灏勮鍒嗘暟(LVEF)(76.97卤3.61vs75.90卤1.67,p?0.05)姝ｅ父,宸紓鏃犵粺璁″鎰忎箟,鑰屽悇鑸掑紶鍔熻兘鎸囨爣鍏锋湁缁熻瀛﹀樊寮，
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