人羊水来源间质干细胞的鉴定及其免疫调节功能和相关机制研究

发布时间：2018-01-19 02:04

本文关键词： 人羊水间质干细胞体外分化颗粒酶B 免疫调节　出处：《实用医学杂志》2013年04期 　论文类型：期刊论文

【摘要】：目的:从人羊水组织中体外培养获得间质干细胞,并探讨其免疫调节能力和相关的调节机制。方法:收集人羊水组织,采用贴壁培养的方法扩增和传代。检测羊水来源的间质干细胞的体外分化能力,并检测其免疫调节能力,同时检测颗粒酶B在人羊水来源间质干细胞中的表达情况,并通过特异性抑制剂阻断方法验证颗粒酶B在其免疫调节中的作用。结果:人羊水来源间质干细胞具有多向分化能力和免疫调节活性,并通过表达颗粒酶B的方式参与其免疫调节功能。结论:体外培养获得了具有免疫调节功能的人羊水来源的间质干细胞,颗粒酶B途径可能是一种新发现的间质干细胞免疫调节方法。
[Abstract]:Objective: to obtain mesenchymal stem cells (MSCs) from human amniotic fluid in vitro and to investigate their immunomodulatory ability and related regulatory mechanisms. Methods: human amniotic fluid tissues were collected. The ability of differentiation of mesenchymal stem cells derived from amniotic fluid in vitro and its immunomodulatory ability were detected by means of adherent culture. At the same time, the expression of granzyme B in human amniotic fluid derived mesenchymal stem cells was detected. The role of granzyme B in the immunomodulation of human amniotic fluid derived mesenchymal stem cells was verified by specific inhibitor blocking method. Results: human amniotic fluid derived mesenchymal stem cells had the ability of multidirectional differentiation and immunomodulatory activity. Conclusion: mesenchymal stem cells derived from human amniotic fluid with immunomodulatory function were obtained in vitro by expression of granzyme B. Granzyme B pathway may be a novel immunoregulatory method for mesenchymal stem cells.
【作者单位】：广州医学院第一附属医院检验系;广州医学院第一附属医院妇产科;
【基金】：国家自然科学青年基金项目(编号:81101730) 广州医学院第一附属医院院级课题(编号:Y201013)
【分类号】：R329
【正文快照】： 间质干细胞(mesenchymal stem cells,MSCs)具有低免疫原性和免疫调节功能[1-3]。因此,间质干细胞在治疗免疫相关性疾病如移植物抗宿主疾病(GVHD)和系统性红斑狼疮(SLE)方面具有重要作用[4-5]。然而,目前对间质干细胞的免疫调节机制并不完全了解,有关报道显示细胞接触性依赖和

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