大鼠原代前脂肪细胞及诱导分化方法的探讨

发布时间：2018-02-04 08:17

本文关键词： 前脂肪细胞原代培养分化　出处：《中国老年学杂志》2013年03期 　论文类型：期刊论文

【摘要】：目的探寻大鼠原代培养前脂肪细胞及诱导分化的最佳方法。方法采用胶原酶消化法体外培养大鼠前脂肪细胞。显微镜下观察细胞形态改变,流式细胞仪检测细胞增殖活性;以分化培养基诱导分化后,油红O染色法进行细胞鉴定。结果成功培养大鼠前脂肪细胞。前脂肪细胞24 h贴壁时为类圆形,3 d后渐成梭形或多角梭形,6 d左右进入指数增长期;流式细胞仪检测证明细胞具有增殖分化能力;油红O染色可见细胞内出现红染颗粒。结论成功建立高效原代培养前脂肪细胞及诱导分化的方法,为脂肪细胞的相关研究提供了良好的细胞模型。
[Abstract]:Objective to explore the best method for primary culture and differentiation of rat preadipocytes. Methods Rat preadipocytes were cultured by collagenase digestion in vitro. The morphological changes of preadipocytes were observed under microscope. Cell proliferation activity was detected by flow cytometry. After differentiation was induced by the differentiation medium, the cells were identified by oil red O staining. Results the rat preadipocytes were successfully cultured. The preadipocytes were round or polygonal after 24 h adherent to the wall for 3 days, and then gradually became fusiform or polygonal fusiform. About 6 days into the exponential growth period; Flow cytometry showed that the cells had the ability of proliferation and differentiation. Oil red O staining showed that red staining granules appeared in the cells. Conclusion High efficient primary cultured adipocytes and induced differentiation were successfully established, which provided a good cell model for the study of adipocytes.
【作者单位】：桂林医学院附属医院呼吸内科;
【基金】：国家自然科学基金资助项目(No.81060009) 广西卫生厅重点科研项目(No.重2010047)
【分类号】：R329
【正文快照】： 阻塞性睡眠呼吸暂停综合征(OSAHS)是一种睡眠呼吸障碍性疾病,发病率逐年升高,已成为危害人类健康的主要疾病之一。关于OSAHS的发病机制目尚未完全明确,但大量研究证实在OSAHS患者中存在严重脂肪代谢紊乱,其中由脂肪细胞分泌的细胞因子不仅调节糖脂代谢,还参与炎症反应、免疫

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