噬菌体展示技术筛选人隐孢子虫P23抗原表位

发布时间：2018-02-07 17:48

本文关键词： 噬菌体展示技术筛选人隐孢子虫 P抗原细胞表位　出处：《中国人兽共患病学报》2013年01期 　论文类型：期刊论文

【摘要】：目的本研究利用噬菌体展示技术筛选人隐孢子虫P23抗原表位,为研制其抗原表位疫苗奠定基础。方法首先用人隐孢子虫重组P23抗原免疫动物以制备抗体,将该抗体与噬菌体展示随机肽库结合,通过ELISA和Western-blot方法鉴定阳性克隆,并对阳性克隆进行增殖,然后对其序列测定和抗原表位预测。结果噬菌体肽库结合抗体后,经ELISA方法可鉴定10株为阳性克隆,Western-blot检测可发现有67kD目的条带出现;序列分析结果可发现它们中具有3个不同结构表位序列,命名为ep1,ep2,ep3,且预测出具有良好的亲水性。结论所得序列是具有B细胞抗原表位。
[Abstract]:Objective to screen the epitopes of human Cryptosporidium P23 antigen by phage display technique in order to establish the foundation for the preparation of the antigen epitope vaccine. Methods the recombinant P23 antigen was first used to immunize animals with human Cryptosporidium p23 antigen to prepare antibodies. The antibody was combined with phage display random peptide library, positive clones were identified by ELISA and Western-blot, and the positive clones were proliferated, then sequenced and epitope predicted. The results of ELISA analysis showed that there were 67kD target bands in 10 positive clones by Western-blot, and three different structural epitopes were found in them by sequence analysis. It was named ep1 ep2 ep3 and was predicted to have good hydrophilicity. Conclusion the obtained sequence is a B cell epitope.
【作者单位】：安徽农业大学;华南农业大学;
【基金】：安徽省高等学校自然科学基金项目(KJ2009B027Z)~~
【分类号】：R382.3

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