布菌Omp31-BLS融合肽抗血清的免疫学性质研究

发布时间：2018-02-08 10:29

本文关键词： 布鲁氏菌 Omp分子 BLS分子抗血清免疫原性　出处：《生物技术》2013年01期 　论文类型：期刊论文

【摘要】：目的:检测Omp31-BLS融合肽抗血清的质量。方法:利用间接酶联免疫吸附实验、免疫双扩散实验以及凝集实验检测Omp31-BLS融合肽制备的抗血清的质量。结果:Omp31-BLS融合肽抗血清可以较好地识别Omp31-BLS融合肽蛋白质,而且,当抗体被稀释4 000倍的时候,酶联免疫吸附实验测定的OD值为1.2,明显高于其他3个对照组。在免疫双扩散实验中,抗血清被稀释64倍的时候,仍然能够出现明显的免疫沉淀线。此外,凝集实验也反映出Omp31-BLS融合肽抗血清可以与Omp31-BLS融合肽抗原发生强烈的凝集作用。结论:Omp31-BLS融合肽抗血清可以较好地识别对应的蛋白质抗原,二者之间存在明显的抗原-抗体反应,为利用Omp31-BLS融合肽进行布鲁氏菌病的血清学诊断提供了理论基础和技术支持。
[Abstract]:Objective: to detect the quality of Omp31-BLS fusion peptide antiserum. Methods: indirect enzyme-linked immunosorbent assay (Elisa) was used. The mass of antiserum prepared by Omp31-BLS fusion peptide was detected by double immunodiffusion assay and agglutination assay. Results: the fusion peptide antiserum of Omp31-BLS fusion peptide can recognize Omp31-BLS fusion peptide protein well, and when the antibody is diluted 4 000 times, The OD value measured by enzyme-linked immunosorbent assay (Elisa) was 1.2, which was significantly higher than that of the other three control groups. In the double immunodiffusion test, when the antiserum was diluted 64 times, the immunoprecipitation line could still appear. The agglutination experiment also showed that the Omp31-BLS fusion peptide antiserum could agglutinate strongly with the Omp31-BLS fusion peptide antigen. Conclusion the fusion peptide antiserum of the Omp31-BLS fusion peptide can recognize the corresponding protein antigen well, and there is obvious antigen-antibody reaction between the fusion peptide antiserum and the Omp31-BLS fusion peptide antiserum. It provides theoretical basis and technical support for serological diagnosis of brucellosis using Omp31-BLS fusion peptide.
【作者单位】：内蒙古科技大学数理与生物工程学院生物工程与技术研究所;
【分类号】：R392

【相似文献】