BMP-2和Noggin在新生小鼠不同部位毛囊发育中的表达

发布时间：2018-02-23 05:38

本文关键词： 毛囊毛囊发育差异骨形态发生蛋白　出处：《生物技术》2013年05期 　论文类型：期刊论文

【摘要】：目的:研究BMP-2与Noggin在新生昆明白小鼠不同皮肤部位毛囊发育差异中的作用。方法:对背部、尾部和触须部皮肤取材,免疫组化检测BMP-2和Noggin的表达分布。结果:背部毛囊发育和尾部相似。头3d都有一个稳态抑制过程,BMP-2在毛囊中强烈表达,Noggin只表达于毛囊上部。4d后背和尾部毛囊发育迅速,BMP-2表达降低,Noggin的表达增强。7d后毛囊发育变缓,BMP-2集中于毛乳头、髓质和外根鞘处。Noggin表达于背皮毛囊毛球上部,尾部整个毛囊表达很强。触须部毛囊发育差异很大,头3d触须处毛囊快速生长,BMP-2在毛囊无表达。Noggin的表达从第2d增强。5d后,BMP-2在整个毛囊中表达,Noggin也持续表达于整个毛囊。结论:不同皮肤的毛囊发育存在差异。BMP-2和Noggin的表达分布的不同对毛囊的维持稳态和生长发育起关键作用,对不同皮肤毛囊的发育的差异也起到了表达调控的作用。
[Abstract]:Objective: to study the role of BMP-2 and Noggin in the development of hair follicles in different skin regions of newborn Kunming white mice. The expression of BMP-2 and Noggin was detected by immunohistochemistry. Results: the development of back hair follicles was similar to that of tail. There was a stable inhibition process of BMP-2 in hair follicles on the first 3 days. The strong expression of noggin in hair follicles was only expressed in the hair follicles of the upper part of the hair follicles. 4 days after the hair follicles developed, the hair follicles developed in the back and tail. The expression of BMP-2 decreased rapidly. The expression of Noggin was enhanced. 7 days later, the development of hair follicles slowed down and BMP-2 was concentrated in the dermal papilla. Noggin was expressed in the upper part of the dorsal hair follicle and the outer root sheath, and the whole hair follicle was strongly expressed in the tail. The development of the hair follicles in the tentacles varied greatly. The expression of BMP-2 in hair follicles was increased from day 2 to day 5. The expression of BMP-2 in hair follicles was also continuously expressed in the whole hair follicle. Conclusion: there are differences in the development of hair follicles between different skin. BMP-2 and Noggin. The difference of expression distribution plays a key role in maintaining stable state and growth and development of hair follicles. It also plays a role in regulating the development of different skin follicles.
【作者单位】：呼和浩特职业学院生物化学工程学院;内蒙古大学生命科学学院;内蒙古医科大学分子生物学研究中心;
【基金】：内蒙古医科大学青年创新基金项目(“小鼠毛囊发育生长中BMP和Noggin基因调控机制的研究”,No.nybq2010cq) 内蒙古自然科学基金项目(“小鼠雄性激素源性脱发模型构建及发病机理的研究”,No.2013MS1122)资助~~
【分类号】：R329

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