慢性高原病大鼠模型FPN、HJV及TMPRSS6水平变化的研究

发布时间：2018-02-25 06:44

  本文关键词： 慢性高原病 膜铁转运蛋白 铁调素调节蛋白 II型跨膜丝氨酸蛋白酶　出处：《青海大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:本课题通过制备CMS大鼠模型,探讨CMS大鼠模型FPN、TMPRSS6及HJV的变化,为揭示CMS铁代谢相关因子的改变,丰富CMS铁代谢的研究内容。方法:选取雄性SD大鼠50只,随机分成常氧组和低氧组,常氧组于西宁地区分别饲养(海拔2260m)7d、15d、30d,各8只大鼠;低氧组于低压氧舱(模拟海拔5000m)分别饲养7d、15d、30d,低氧7d 8只大鼠,低氧15d、30d各9只大鼠。分别于饲养7d、15d、30d测定大鼠外周血血常规,q RT-PCR法测定大鼠肝脏FPN、肝脏m HJV、肝脏TMPRSS6、肌肉s HJV m RNA表达水平,Western blotting法定肝脏m HJV、肝脏TMPRSS6、肌肉s HJV蛋白水平,IHC法测大鼠肝脏FPN蛋白水平。结果:(1)大鼠肝脏FPN m RNA表达水平,低氧7d、低氧15d、低氧30d均高于各常氧对照组(均P0.01);低氧组内比较低氧15d、低氧30d均高于低氧7d(均P0.01)。(2)大鼠肝脏FPN蛋白水平,低氧7d和低氧30d均高于各常氧对照组(均P0.01),低氧15d高于常氧15d(P0.05);低氧组内比较低氧15d、低氧30d均高于同组7d(均P0.01),低氧30d高于同组15d(P0.05)。(3)大鼠肝脏m HJV m RNA表达水平,低氧15d、低氧30d均低于各自常氧对照组(均P0.01);低氧组内比较低氧15d、低氧30d均低于低氧7d(P0.05及P0.01),低氧30d低于低氧15d(P0.05)。(4)大鼠肌肉s HJV m RNA表达水平,低氧7d、低氧15d、低氧30d均高于各自常氧对照组(均P0.01)。(5)大鼠肝脏TMPRSS6 m RNA表达水平,低氧7d、15d、30d均高于各自常氧对照组(均P0.01);低氧组内比较低氧30d高于低氧7d及15d(均P0.01)。(6)大鼠肝脏m HJV蛋白表达水平,低氧7d、15d、30d比各常氧对照组降低(均P0.01),随缺氧时间延长m HJV含量逐渐降低。(7)大鼠肌肉s HJV蛋白表达水平,低氧7d、15d、30d较常氧对照组升高(P0.05),且随着缺氧时间延长s HJV表达逐渐增加。(8)大鼠肝脏TMPRSS6蛋白表达水平,低氧7d、15d、30d比各常氧对照组升高(均P0.01),随缺氧时间延长TMPRSS6表达逐渐升高。结论:在高原低压低氧条件下,FPN、s HJV、TMPRSS6 m RNA和蛋白表达水平增加,并随缺氧时间延长逐渐增加,m HJV m RNA和蛋白表达水平减低,并随缺氧时间延长逐渐降低。FPN、m HJV、s HJV、TMPRSS6参与CMS铁代谢的调节,可能是CMS铁代谢调节的重要靶点。
[Abstract]:Objective: to investigate the changes of CMS rat model and HJV in order to reveal the changes of CMS iron metabolism related factors and enrich the research contents of CMS iron metabolism. Methods: 50 male Sprague-Dawley rats were selected. They were randomly divided into normoxic group and hypoxic group. The normoxic group was fed in Xining area (2260mg / 7d / 15d / 30d, n = 8), hypoxia group was fed in a hypobaric oxygen chamber (simulated altitude 5000m) for 7d / 15d / 30d, hypoxia group (n = 8) for 7 days, respectively, and the hypoxia group (n = 8) were fed with hypoxia for 7 days (n = 8) and hypoxia group (n = 8) for 7 days. The expression levels of liver FPNs, liver mHJVs, liver TMPRSS6, muscle s HJV m RNA, liver TMPRSS6, liver TMPRSS6, liver TMPRSS6 and muscle s HJV protein water were determined by qq RT-PCR assay in peripheral blood of rats fed with hypoxia for 15 days and 30 days, respectively. Results the expression of FPN m RNA in the liver of rats was determined by using the method of flat IHC, and the expression of FPN m RNA in the liver of rats was determined. The levels of FPN protein in the liver of rats in hypoxia group were higher than those in normal control group (all P0.01D), and those in hypoxic group were higher than those in hypoxic group (P0.01g / kg) for 7d, 15d and 30d respectively. The expression of m HJV m RNA in the liver of rats in hypoxic group was higher than that in the control group at 15 days after hypoxia for 15 days and 30 days after hypoxia compared with that of the control group for 7 days (all P0.01 and 30 days of hypoxia were higher than that in the same group at 15 days P0.05. 5. 3), and the expression of m HJV m RNA in the liver of rats in hypoxia group was higher than that in the control group at 15 days after hypoxia (all P0.01 and 30 days of hypoxia was higher than that in the same group at 15 days P0.05. 0. 3). After 15 days of hypoxia, 30 days of hypoxia were lower than that of the control group (P 0.01), and the expression of HJV m RNA was lower in hypoxia group than in hypoxia group for 15 days, hypoxia for 30 days, and hypoxia for 30 days lower than that of hypoxia for 15 days (P0.05, P 0.05, P 0.01), and for 30 days, the expression level of HJV m was lower than that in hypoxic group for 15 days, while that in hypoxia group for 30 days was lower than that in hypoxia group for 15 days. The expression level of TMPRSS6 m RNA in the liver of rats exposed to hypoxia for 7 days, 15 days and 30 days was higher than that in the control group (P0.01. 05). The expression of m HJV protein in the liver of rats in hypoxia group was higher than that in hypoxia group for 7 days and 15 days after hypoxia (P 0.01) and the expression of m HJV protein in the liver of hypoxia group was higher than that of normal control group (P 0.01) for 30 days, and the expression level of m HJV protein was higher in hypoxia group for 30 days than that in hypoxia group for 7 days and 15 days. The expression level of s HJV protein in the muscle of rats was decreased after hypoxia for 7 days and 15 days after hypoxia compared with the control group (P0.01 for all, and the content of m HJV decreased gradually with the prolongation of hypoxia time.) the expression level of s HJV protein in the muscle of rats was lower than that of the control group. Compared with the control group, the expression of TMPRSS6 protein in the liver of rats increased gradually with the prolongation of hypoxia time, and the expression of s HJV increased gradually after hypoxia for 7 days and 15 days, 30 days after hypoxia compared with the control group, and the expression of TMPRSS6 protein in the liver of the rats increased gradually with the prolongation of hypoxia. Compared with the control group, the expression of TMPRSS6 increased gradually with the prolongation of hypoxia time. Conclusion: the expression level of TMPRSS6 m RNA and protein in HJV is increased under low pressure and hypoxia condition at high altitude. With the prolongation of hypoxia time, the expression of HJV m RNA and protein decreased gradually, and with the prolongation of hypoxia time, TMPRSS6 was involved in the regulation of iron metabolism of CMS, which may be an important target for the regulation of CMS iron metabolism.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R594.3;R-332

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