缺失mir122抑制斑马鱼肝脏前体细胞向肝细胞分化

发布时间：2018-02-25 23:19

本文关键词： mir 肝脏前体细胞细胞分化肝脏斑马鱼　出处：《遗传》2013年04期 　论文类型：期刊论文

【摘要】：作为机体内最大的脏器之一,肝脏对于机体的新陈代谢、解毒作用以及内环境的稳定有着重要的作用。FGF、BMP以及WNT信号通路及众多转录因子形成的基因调节网络精密调控着肝脏的发育,然而小分子RNA在肝脏形成中的功能却所知甚少。近年来的研究发现,mir122在肝细胞中高表达,对于维持肝脏的代谢功能起着至关重要的作用,但是其在肝脏发育中的功能还不清楚。为了深入探讨mir122在斑马鱼(Danio rerio)胚胎发育中的作用,文章首先检测了mir122在发育过程中的时空表达,发现mir122特异表达在肝脏,并且在肝脏前体细胞向肝细胞分化过程中表达水平显著增高。文章还通过用反义核苷酸Morpholino(MO)敲降mir122的表达,发现敲降mir122对肝脏前体细胞的起源、肝原基的形成以及肝脏前体细胞的增殖没有明显的影响,但是,mir122的缺失导致肝脏前体细胞不能分化为肝细胞。因此,mir122不仅参与了成体肝脏代谢功能的调节,还在肝脏前体细胞向肝细胞分化过程中不可或缺。
[Abstract]:As one of the largest organs in the body, liver metabolism, Detoxification and stability of internal environment play an important role. The development of liver is precisely regulated by WNT signaling pathway and gene regulatory network formed by many transcription factors. However, little is known about the function of small molecule RNA in liver formation. Recent studies have found that the high expression of RNA 122 in liver cells plays a crucial role in maintaining the metabolic function of the liver. In order to investigate the role of mir122 in the embryonic development of Danio Rerio, the expression of mir122 during the development of the liver was detected, and the expression of mir122 was found to be specific in the liver. The expression level of mir122 was significantly increased during the differentiation of liver precursor cells into hepatocytes. The origin of knockdown mir122 on liver progenitor cells was also found by using antisense nucleotide Morpholino motilla to reduce the expression of mir122. The formation of hepatic primordium and the proliferation of liver progenitor cells have no obvious effect, but the absence of liver-precursor cells leads to the inability of liver progenitor cells to differentiate into hepatocytes. Therefore, Ximir122 is not only involved in the regulation of the metabolic function of adult liver. It is also indispensable in the differentiation of liver precursor cells into hepatocytes.
【作者单位】：中国科学院动物研究所生物膜与膜生物工程国家重点实验室;河北省安国市医院外科门诊;
【基金】：国家重点基础研究发展计划(973计划)项目(编号:2011CBA01101) 国家自然科学基金项目(编号:30971656)资助
【分类号】：R363

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