轮状病毒LLR株在两种不同细胞中病毒滴度的比较

发布时间：2018-03-01 02:08

本文关键词： 轮状病毒LLR株牛肾细胞 Vero细胞病毒滴度　出处：《中国新药杂志》2013年06期 　论文类型：期刊论文

【摘要】：目的:通过轮状病毒LLR株在牛肾细胞和Vero细胞中培养效果的比较,为轮状疫苗的生产筛选最佳的细胞基质和培养条件。方法:将轮状病毒LLR株按MOI 0.02分别接种牛肾细胞和Vero细胞,在相同培养条件下进行培养,每天观察两种细胞病变的情况,同时抽样检测病毒滴度,分析两种细胞对轮状病毒LLR株的敏感性。结果:牛肾细胞在感染轮状病毒LLR株后d 3病毒滴度达到最高,为6.8 lgCCID50.mL-1;而Vero细胞在感染轮状病毒LLR株后d 8病毒滴度达到最高,为7.3 lgCCID50.mL-1。轮状病毒LLR株在牛肾细胞和Vero细胞上均能达到满意的病毒表达量。结论:轮状病毒LLR株在Vero细胞中培养能够达到理想的病毒表达量,利用Vero细胞培养轮状病毒LLR株,有利于提高疫苗的产量和质量。
[Abstract]:Objective: to compare the effects of rotavirus LLR in bovine kidney cells and Vero cells. Methods: bovine kidney cells and Vero cells were inoculated with rotavirus LLR strain according to MOI 0.02, and cultured under the same culture conditions. At the same time, the virus titer was detected by sampling, and the sensitivity of the two cells to rotavirus LLR strain was analyzed. Results: the titer of bovine kidney cells reached the highest on day 3 after infection with rotavirus LLR strain. The titer of Vero cells reached the highest on d 8 after infection with rotavirus LLR strain. Rotavirus LLR strain can achieve satisfactory viral expression in bovine kidney cells and Vero cells. Conclusion: rotavirus LLR strain can achieve ideal viral expression in Vero cells, and rotavirus LLR strain can be cultured by Vero cells. It is beneficial to improve the quantity and quality of vaccine.
【作者单位】：兰州生物制品研究所有限责任公司;
【分类号】：R373

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