大鼠背部跨区皮瓣的Choke静脉的变化规律

发布时间：2018-02-28 22:05

本文关键词： 皮瓣 Choke血管皮窗血流动力学大鼠　出处：《解剖学报》2013年02期 　论文类型：期刊论文

【摘要】：目的通过皮窗对大鼠背部跨区皮瓣Choke静脉的变化情况进行观察。方法首先利用氧化铅-明胶一次性整体血管灌注技术,对30只SD大鼠背部血管的分布情况进行研究;然后在10只SD大鼠背部一侧切取大小为10.0cm×3.5cm的跨区皮瓣,放置皮窗,用体视显微镜连续观察8d并摄片记录Choke动静脉的变化情况。另2只大鼠同法安置皮窗后,尾静脉注射2%伊文思蓝各1ml,视频记录Choke区动静脉的循环过程。结果跨区皮瓣切取后,Choke区的动脉与静脉管径均扩张,连接小静脉于术后(60±12)h开始出现管径扩张,一直延续到(4.5±0.5)d。通过尾静脉注射伊文思蓝,可以完整地观察到Choke动静脉的循环过程,其动脉血流速度为2.53mm/s。结论在皮瓣切取后的早期,静脉血主要以"迷宫"式回流为主,而术后晚期则通过"迷宫式"及"瓣膜失效"两种方式进行回流,以"瓣膜失效"为主。伊文思蓝可作为一种血流示踪剂,与皮窗直视观测相结合,适用于皮瓣内的微循环血流动力学研究。
[Abstract]:Objective to observe the changes of Choke veins in dorsal flap of rats by skin window. Methods the distribution of dorsal blood vessels in 30 SD rats was studied by using the technique of one-off global vascular perfusion with lead oxide and gelatin. Then 10 Sprague-Dawley rats were cut off from the back side of the skin flap with the size of 10.0cm 脳 3.5cm, and the skin window was placed. The changes of Choke arteriovenous vessels were recorded by stereoscopic microscope for 8 days. The other two rats were treated with the same method. 2% Evans blue was injected into the tail vein, and the circulation process of arteriovenous in Choke area was recorded by video recording. Results the arterial and venous diameters of the choke area were both dilated after transregional flap resection, and the diameter of the connecting small vein began to dilate at 60 卤12 hours after operation. The circulation process of Choke artery and vein was observed completely by injecting Evans blue into caudal vein, and the arterial blood flow velocity was 2.53 mm / s. Conclusion in the early stage after flap resection, the main venous blood flow was labyrinth reflux. In the later stage of operation, "labyrinth" and "valve failure" were reflux, mainly "valve failure". Evans blue can be used as a blood tracer and combined with direct observation of skin window. It is suitable for the study of microcirculation hemodynamics in the skin flap.
【作者单位】：承德医学院解剖学教研室;温州医学院解剖学教研室;福建医科大学解剖学教研室;温州医学院附属义乌医院手足外科;
【基金】：浙江省科技厅资助项目(2010C33012)
【分类号】：R322

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