构建斑马鱼心脏损伤-再生模型的手术方法

发布时间：2018-03-01 16:00

本文关键词： 斑马鱼心脏手术切除再生　出处：《遗传》2013年04期 　论文类型：期刊论文

【摘要】：斑马鱼心脏再生是近年来心血管再生医学研究的新热点之一,也是以斑马鱼为模式进行脊椎动物遗传发育研究的一个新的重要方向。通过了解斑马鱼成体心脏再生的过程和研究其分子和细胞机制有可能为诱导哺乳动物成体心脏再生、治疗心肌梗塞等人类心脏疾病提供理论依据。文章主要介绍通过简单的手术切除成体斑马鱼约20%心室造成成体心脏损伤、诱导心脏再生的操作方法与经验。其基本流程主要包括麻醉成鱼、在体视镜下用尖镊撕开斑马鱼心脏腹面的皮肤和心包膜以暴露心脏、用剪刀切除心尖区域的部分心室。这种方法的手术成功率可达90%以上,操作简便且重复性好,是目前研究斑马鱼成体心脏损伤-再生的最常用的方法。
[Abstract]:The heart regeneration of zebrafish is one of the new hotspots in cardiovascular regenerative medicine in recent years. It is also a new important research direction of vertebrate genetic development based on zebrafish. By understanding the process of adult heart regeneration in zebrafish and studying its molecular and cellular mechanism, it is possible to induce adult heart regeneration in mammals. The treatment of human heart diseases such as myocardial infarction provides a theoretical basis. This article mainly introduces the simple surgical excision of adult zebrafish ventricular injury caused by about 20% adult heart. Methods and experience in inducing cardiac regeneration. The basic procedures include anaesthesia of adult fish and the opening of skin and pericardium on the ventral surface of zebrafish heart with sharp forceps under stereoscopic microscope to expose the heart. Scissors were used to remove some ventricles in the apical region of the heart. The surgical success rate of this method is more than 90% and the operation is simple and reproducible. It is the most commonly used method to study the injury and regeneration of adult zebrafish heart at present.
【作者单位】：北京大学生命科学学院细胞增殖与分化教育部重点实验室;
【基金】：国家重大科学研究计划项目(编号:2012CB945101) 国家自然科学基金项目(编号:31110103904)资助
【分类号】：R-332

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