两种地霉对甲板侵袭力的分析

发布时间：2018-03-14 09:34

本文选题：白地霉　切入点：林生地霉　出处：《中国皮肤性病学杂志》2013年06期 　论文类型：期刊论文

【摘要】：目的通过建立真菌透甲模型,探讨林生地霉、白地霉在体外能否侵犯健康甲板。方法以红色毛癣菌作为阳性对照,制备透甲模型,分为林生地霉组、白地霉组和红色毛癣菌组,观察并记录各组透甲时间,采用统计学方法比较是否存在差异性。采用扫描电镜观察受侵甲板的微观变化。结果三株真菌的菌丝均能穿透甲板,其中林生地霉组的透甲时间为(15.07±3.28)d,白地霉组为(14.50±1.67)d,红色毛癣菌组为(6.76±1.72)d。林生地霉组和白地霉组的透甲时间均明显长于红色毛癣菌组,差异有统计学意义(P0.05),林生地霉组和白地霉组的透甲时间差异无统计学意义(P0.05)。扫描电镜观察到三种菌株均有菌丝从角化细胞缝隙及细胞中央穿过,相对于健康甲板,受侵甲板背侧面角化细胞"鱼鳞样"排列消失,边缘模糊,细胞间隙宽窄不等,腹侧面角化细胞未见明显变化。结论林生地霉和白地霉均能穿透甲板,但侵袭力较红色毛癣菌弱。
[Abstract]:Objective to establish a fungus through armour model, and to investigate whether Trichophyton woodland and Trichophyton albicans can invade the health deck in vitro. Methods the model was made by using Trichophyton rubrum as positive control, and was divided into three groups: dipyridium forest group, Trichophyton albicans group and Trichophyton rubrum group. The time of penetration was observed and recorded, and the difference was compared by statistical method. The microscopic changes of the invading deck were observed by scanning electron microscope. Results the mycelium of the three fungi could penetrate the deck. The time of nail penetration was 15.07 卤3.28 days in Trichoderma forest group, 14.50 卤1.67 days in Trichophyton albicans group and 6.76 卤1.72 days in Trichophyton rubrum group. The penetration time of Trichophyton rubrum group and Trichophyton albicans group was significantly longer than that of Trichophyton rubrum group. The difference was statistically significant (P 0.05), but there was no significant difference in the penetration time between the two groups. Scanning electron microscope showed that the mycelium passed through the keratinocyte crevice and the center of the cell, as opposed to the healthy deck. The "ichthyosis" of keratinocytes in the dorsal side of the invaded deck disappeared, the edges were blurred, the intercellular space was not narrow, and the keratinocytes of the ventral side did not change obviously. Conclusion both Trichoderma plantarum and Dipyridium albicans can penetrate the deck. But the invasiveness was weaker than that of Trichophyton rubrum.
【作者单位】：河北医科大学第四医院皮肤科;承德医学院附属医院皮肤科;
【分类号】：R379.9

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