REGγ对生发中心反应的调控研究

发布时间：2018-03-14 12:06

本文选题：REGγ　切入点：生发中心　出处：《华东师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：生发中心是一种存在于外周淋巴器官中的亚结构,B细胞在生发中心经历扩增,突变,筛选以及分化等一系列过程最终形成浆细胞或记忆细胞。虽然人们对生发中心反应的过程和机理已经有了一定了解,但是其中具体的分子调控机理并不清楚。本文首次发现一种20S蛋白酶体激活因子REGy对生发中心反应调控具有重要作用。研究表明,REGy缺失会导致小鼠血清中抗原特异性抗体水平显著下降。对人扁桃体B细胞各亚群的检测发现,REGy只在生发中心B细胞中特异性高表达。对B细胞发育成熟过程的检测揭示了 REGy缺失并不影响该过程的正常进行,并且正常状态下REGy敲除小鼠派氏结中生发中心B细胞比例并未受到严重影响。对小鼠使用经典抗原免疫后发现,REGy并不影响生发中心的建立和维持,但无论是初次免疫还是二次免疫,REGy敲除小鼠抗体分泌量显著降低,并且在CIA模型中也证实了这一影响。我们推测REGy可能是通过影响浆细胞的功能导致这一现象的产生,但其中具体的分子机制以及这种现象是否是由B细胞本身引起的还不清楚。总而言之,本研究证实REGγ参与了生发中心反应调控,并且REGy缺失能够缓解CIA模型中小鼠关节炎的症状。
[Abstract]:Germinal center is a substructural B cell in peripheral lymphoid organs that has been amplified and mutated in the germinal center. A series of processes, such as screening and differentiation, eventually form plasmacytes or memory cells. However, the specific molecular regulation mechanism is not clear. This paper first found that a 20s proteasome activator REGy plays an important role in the regulation of germinal center reaction. The detection of subsets of human tonsil B cells showed that REGy was only specifically expressed in germinal center B cells. The detection of the maturation of B cells revealed that the absence of REGy did not affect the expression of the protein. The normal progress of the process, The percentage of germinal center B cells in REGy knockout mice was not seriously affected under normal condition. It was found that the establishment and maintenance of germinal centers were not affected by the immunization of mice with classical antigens. However, both primary and secondary immunizations showed a significant decrease in antibody secretion in CIA knockout mice, and this effect was confirmed in CIA model. We speculate that REGy may result in the production of this phenomenon by affecting the function of plasma cells. But the specific molecular mechanism and whether the phenomenon is caused by B cells is not clear. In a word, REG 纬 is involved in the regulation of germinal center reaction. And REGy deletion alleviates the symptoms of arthritis in mice in the CIA model.
【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R392

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