钙离子在肿瘤坏死因子α诱导心肌肥大中的作用

发布时间：2018-03-20 11:34

本文选题：心肌肥大　切入点：肿瘤坏死因子α　出处：《中国动脉硬化杂志》2013年01期 　论文类型：期刊论文

【摘要】：目的研究Ca2+在肿瘤坏死因子α(TNF-α)诱导心肌细胞肥大中的作用。方法 Lowry法测心肌细胞蛋白含量;计算机图像分析系统测心肌细胞体积;3H-亮氨酸掺入法测心肌细胞蛋白合成;Till阳离子测定系统观察胞内[Ca2+]i瞬变。结果 IP3R阻断剂2-APB(30μmol/L)或RyR阻断剂ryanodine(50μmol/L)能降低由TNF-α(100μg/L)诱导的心肌细胞蛋白合成、蛋白含量以及细胞体积增加,二者合用抑制作用更强。L型Ca2+通道阻断剂nifedipine(50μmol)对上述反应无明显作用(P0.05)。IP3R阻断剂2-APB(30μmol/L)或RyR阻断剂ryanod-ine(50μmol/L)能降低由TNF-α(100μg/L)诱导的心肌细胞内钙离子瞬变幅度增高,二者合用作用抑制更强。L型Ca2+通道阻断剂nifedipine(50μmol/L)对其无明显作用(P0.05)。结论 TNF-α通过调节心肌细胞内钙离子浓度从而诱导心肌细胞肥大。而在此过程中TNF-α可能主要是通过作用IP3R和RyR促使胞内钙贮库Ca2+释放而引起胞内钙离子水平增高的,而非通过打开L型Ca2+通道。
[Abstract]:Objective to study the role of Ca2 in cardiomyocyte hypertrophy induced by tumor necrosis factor- 伪 (TNF- 伪). Methods the protein content of cardiomyocytes was measured by Lowry method. A computerized image analysis system was used to measure the volume of cardiomyocytes by 3H-leucine incorporation method. The intracellular [Ca2] I transient was observed by the Till cationic assay system. Results IP3R blockers 2-APBN 30 渭 mol / L) or RyR blockers ryanodine(50 渭 mol / L) could be reduced to 100 渭 g / L induced by TNF- 伪 (100 渭 g / L). Protein synthesis in cardiomyocytes, The protein content and cell volume increased. The combination of nifedipine(50 渭 mol, a more potent inhibitor of Ca2 channel, had no obvious effect on the above reaction. P0.05N. IP3R blocker 2-APBN 30 渭 mol / L) or RyR blocker ryanod-ine(50 渭 mol / L) could decrease the transient increase of Ca ~ (2 +) induced by TNF- 伪 ~ (100 渭 g / L) in cardiomyocytes. The combination of TNF- 伪 and TNF- 伪 can inhibit the hypertrophy of cardiomyocytes by regulating the concentration of calcium ions in cardiomyocytes, and TNF- 伪 may be mainly induced by TNF- 伪 during the course of the inhibition of nifedipine(50 渭 mol / L, a stronger antagonist of Ca2 channel. The effect of IP3R and RyR on the release of Ca2 from intracellular calcium storage resulted in the increase of intracellular calcium level. Instead of opening L-type Ca2 channels.
【作者单位】：辽宁医学院附属第一医院;辽宁医学院药理学教研室;
【基金】：辽宁省教育厅项目(L2010304,2009A455) 辽宁省科技厅计划项目(2010225034)
【分类号】：R363

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