瑞舒伐他汀对同型半胱氨酸损伤的人脐静脉内皮细胞超氧化物歧化酶活性和丙二醛含量的影响

发布时间：2018-03-22 03:15

本文选题：瑞舒伐他汀　切入点：内皮细胞　出处：《中国动脉硬化杂志》2013年09期 　论文类型：期刊论文

【摘要】：目的探讨瑞舒伐他汀对同型半胱氨酸(Hcy)损伤的人脐静脉内皮细胞(HUVEC)超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。方法原代培养HUVEC,2~3代进行实验,用一定浓度Hcy损伤HUVEC建立细胞损伤模型,然后瑞舒伐他汀干预研究,采用黄嘌呤氧化酶法测定SOD的活性,硝酸还原酶法检测细胞上清的一氧化氮(NO)含量,硫代巴比妥酸法检测MDA的水平;Real-time PCR检测SOD1 mRNA表达的变化。结果 Hcy刺激24 h SOD1 mRNA表达量、NO、SOD浓度较空白对照纽显著降低,MDA含量较空白对照组显著升高(P0.05);瑞舒伐他汀预处理2 h后再加入等浓度Hcy,刺激24 h后SOD1 mRNA表达量、NO、SOD浓度显著升高,MDA含量显著下降(P0.05)。结论瑞舒伐他汀可通过改善SOD活性和减低MDA水平发挥抗氧化作用,且这种作用独立于它的降脂作用。
[Abstract]:Objective To observe the effect of rosuvastatin on homocysteine (Hcy) induced human umbilical vein endothelial cells (HUVEC) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. The cultured HUVEC, 2~3 generation experiment, with a certain concentration of Hcy damage in HUVEC cell injury model established. Then the rosuvastatin intervention study, SOD was determined by the method of xanthine oxidase activity, nitric oxide cell supernatant nitrate reductase assay (NO) detection of MDA content, the level of thiobarbituric acid method; change of Real-time PCR to detect the expression of SOD1 mRNA Hcy 24 h SOD1 mRNA. The expression of NO, stimulate, SOD concentration compared with the blank the control group was significantly decreased, MDA content increased significantly compared with control group (P0.05); rosuvastatin pretreatment for 2 h after adding concentration of Hcy, after 24 h stimulation of SOD1 expression of mRNA, NO, SOD concentration significantly increased, MDA content decreased significantly (P0.05). Conclusion rosuvastatin can improve SOD activity and reduce MDA levels play a role in anti-oxidation, and this effect is independent of its lipid-lowering effect.

【作者单位】：中山大学附属第三医院心内科;
【分类号】：R363

【参考文献】