膜性肾病小鼠模型的建立与鉴定

发布时间：2018-03-24 23:11

本文选题：膜性肾病　切入点：阳离子牛血清白蛋白　出处：《细胞与分子免疫学杂志》2013年02期

【摘要】：目的建立小鼠膜性肾病模型。方法取6周龄健康雄性BALB/c小鼠20只,分为对照组和模型组。模型组小鼠皮下注射0.2 mg阳离子牛血清白蛋白(C-BSA)和等体积的完全弗氏佐剂,对照组皮下注射生理盐水和完全弗氏佐剂。2周后,模型组小鼠尾静脉注射阳离子牛血清白蛋白,每周3次,隔日注射,对照组使用生理盐水代替。模型的鉴定则采用血液生化检查和病理检查(PASM染色、IgG免疫荧光染色、透射电镜)等方法。结果 8周后12只模型组小鼠出现高蛋白尿、低蛋白血症和高胆固醇血症;PASM染色显示出类似于早期膜性肾病,IgG免疫荧光染色显示出沿毛细血管壁呈颗粒状物沉积,透射电镜下有足突的广泛融合。结论 C-BSA尾静脉注射小鼠可成功构建小鼠膜性肾病模型。
[Abstract]:Objective to establish a model of membranous nephropathy in mice. Methods Twenty healthy male BALB/c mice aged 6 weeks were divided into two groups: control group and model group. Mice in the model group were subcutaneously injected with 0.2 mg cationic bovine serum albumin (BSA) and complete Freund's adjuvant of equal volume. In control group, normal saline and complete Freund's adjuvant were injected subcutaneously for 2 weeks, and cationic bovine serum albumin was injected into tail vein of model group three times a week, every other day. In the control group, physiological saline was used instead. The model was identified by blood biochemical examination and pathological examination, PASM immunofluorescence staining, transmission electron microscopy, etc. Results after 8 weeks, 12 mice in the model group developed hyperproteinuria. PASM staining of hypoproteinemia and hypercholesterolemia showed granular deposition along the capillary wall similar to that of early membranous nephropathy. Conclusion the mouse model of membranous nephropathy can be successfully constructed by tail vein injection of C-BSA.
【作者单位】：第四军医大学基础部病理学教研室;
【基金】：国家自然科学基金(81170798,81070249)
【分类号】：R692.3;R-332

【二级参考文献】