白介素17的进化及结构分析

发布时间：2018-03-26 03:51

本文选题：白介素　切入点：比较基因组学　出处：《第二军医大学学报》2013年01期

【摘要】：目的从系统发生学和结构生物信息学的角度探讨脊椎动物白介素17(IL-17)基因的起源及其蛋白质结构。方法从美国国家生物技术信息中心(NCBI)及联合基因组研究所(JGI)数据库搜索得到全部已知的人、斑马鱼和文昌鱼IL-17蛋白质序列,同时从另外几个数据库中搜索得到鸡、海鞘等7种常见模式生物的部分IL-17蛋白质序列。利用MEGA软件为这些蛋白质序列构建系统发生树,并用MODELLER软件预测斑马鱼、文昌鱼、海鞘、线虫IL-17的三维结构,再与已有的人IL-17F晶体结构进行比较分析。结果虽然所有IL-17蛋白质序列中都含有4个保守的可能形成一个"半胱氨酸结"构象的半胱氨酸残基,但文昌鱼展示出两种不同的分布模式,其中一种与脊椎动物的分布模式相似,另一种是文昌鱼自身特有。系统发生树显示,脊椎动物IL-17基因的直系同源(ortholog)在文昌鱼基因组中没有出现,而出现于海鞘基因组中。IL-17蛋白质三维模型的比较结果与系统发生树一致。结论文昌鱼的IL-17可能是IL-17进化的分歧点,脊椎动物IL-17基因的直系同源可能最早出现于尾索动物。
[Abstract]:The purpose of learning and structural bioinformatics systems from the perspective of the study of vertebrate interleukin 17 (IL-17) gene and its protein structure origin. Methods from the National Center for Biotechnology Information (NCBI) and the Joint Genome Research (JGI) database search to get all known human, zebrafish and amphioxus IL-17 protein sequences of chicken search from several other databases at the same time, part of the IL-17 protein sequences of 7 kinds of ascidian model organisms. These protein sequences for phylogenetic tree was constructed using MEGA software and the MODELLER software is used to predict the zebrafish, amphioxus, ascidians, three-dimensional structure of nematode IL-17, then compared with IL-17F crystal structure. The cysteine although the results of all residues in the IL-17 protein sequence contains 4 conserved may form a cysteine knot conformation, but show amphioxus Two different distribution patterns, including a similar distribution pattern and vertebrate, the other is unique. Amphioxus phylogenetic tree showed that vertebrate orthologous IL-17 gene (ortholog) does not appear in the amphioxus genome, tree consistent occurrence comparison results appeared in.IL-17 gene protein in ascidian 3D model and group the system is IL-17. Conclusion the amphioxus IL-17 bifurcation point of evolution, vertebrate IL-17 gene orthology may first appeared in a animal.

【作者单位】：第二军医大学基础部生物物理学教研室;山东卫生报刊社《山东医药》编辑部;山东大学山东省口腔生物重点实验室;山东大学口腔医院口腔颌面外科;
【基金】：山东省科技发展计划(2012G0021833)~~
【分类号】：R392.1

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