急性抗体介导排斥反应中微血栓形成对移植物损伤的机制研究

发布时间：2018-03-31 06:26

本文选题：三磷酸核苷双磷酸水解酶　切入点：B细胞　出处：《中国免疫学杂志》2013年04期

【摘要】：目的:探讨急性抗体介导排斥反应(Antibody-mediated rejection,AMR)中微血栓形成对移植器官损伤的效应及机制。方法:在三磷酸核苷双磷酸水解酶(NTPDase1)野生型裸鼠皮肤移植模型上诱导急性AMR,用实时荧光定量PCR、倒置显微镜等方法,研究B细胞NTPDase1的表达活性、移植皮肤NTPDase1 mRNA表达量、血小板活化标记物和血小板移动平均速率测定值,及移植皮肤病理变化;补充外源性NTPDase1预处置对血小板活化和移植皮肤损伤的影响。结果:急性AMR发生时,B细胞NTPDase1的表达上调,移植皮肤的NTPDase1 mRNA表达下降,血小板活化、移植皮肤受损;NTPDase1预处置可有效抑制血小板活化、保护移植皮肤。结论:NTPDase1降解胞外ADP失衡可能是急性AMR中移植物损伤的主要原因,中剂量外源性NTPDase1预处置保护移植物有效、安全。
[Abstract]:Objective: to investigate the effect and mechanism of microthrombus formation on organ injury in antibody-mediated rejection AMRs. Methods: acute AMRs were induced by NTPDase1, a ribonucleoside diphosphate hydrolase (NTPDase1), in nude mice. Using real-time fluorescence quantitative PCR, inverted microscope and other methods, To study the expression activity of NTPDase1 in B cells, the expression of NTPDase1 mRNA in transplanted skin, the measurement of platelet activation marker and average rate of platelet movement, and the pathological changes of transplanted skin. Results: during the course of acute AMR, the expression of NTPDase1 in B cells was up-regulated, the expression of NTPDase1 mRNA in transplanted skin was decreased, and the platelet was activated. Conclusion the imbalance of extracellular ADP degradation by 1% NTPDase1 may be the main cause of graft injury in acute AMR and the protective effect of middle dose exogenous NTPDase1 on graft is effective and safe.
【作者单位】：首都医科大学附属北京天坛医院;
【基金】：国家自然科学基金资助项目(81270838) 北京市自然科学基金资助目(7123213)资助
【分类号】：R392.11

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