缺血后处理对供体犬肺组织细胞凋亡基因表达的影响

发布时间：2018-04-14 08:31

本文选题：肺移植 + 缺血后处理　；参考：《中山大学学报(医学科学版)》2013年01期

【摘要】：【目的】观察供体犬肺再灌注后早期供体肺组织脂质过氧化反应的变化,了解缺血后处理对供体犬肺植入后细胞凋亡基因表达的变化情况。【方法】随机选取12对比咯犬,组成供受体,进行异体左侧单肺移植术。随机分成两组,对照组:6对犬,进行供受体左侧异体单肺移植,不予缺血后处理的干预,按常规方式进行。缺血后处理组:6对犬,进行供受体左侧异体单肺移植,常规方式获取的供体犬肺植入后,再灌注早期实施3个周期的10 s再灌～10 s再阻断,总时程1 min的缺血后处理。于供体犬肺再灌注后1、2 h时间点采集供肺标本检测超氧化物歧化酶(SOD)的活性、丙二醛(MDA)的含量;采用RT-PCR技术检测供体肺脏再灌注后1、2 h时间点细胞凋亡基因Bcl-2、Bax的表达;光镜下观察供体犬肺植入后1、2 h时间点肺组织的病理变化,应用SPSS 13.0统计软件处理,以P0.05为统计学上有显著差别。【结果】供体肺脏植入时间平均(35.9±1.7)min。缺血后处理组在1、2 h时间点的供体肺组织SOD活性水平较对照组升高、MDA含量较对照组减低,有统计学差异(P0.05);对照组与实验组相比较,肺组织再灌注后1、2 h时间点,Bc12蛋白表达上调(P0.05),而Bax蛋白表达下调(P0.05);缺血后处理组肺组织光镜下观察在各时间点的炎症反应均较对照组的变化轻微。【结论】缺血后处理可以抑制再灌注时氧化自由基的堆积,从而减少供体肺的缺血再灌注损伤;缺血后处理能够通过抑制促凋亡Bax蛋白的表达,及促进抗凋亡Bc12蛋白表达,从而影响内源性细胞凋亡途径。
[Abstract]:[objective] to observe the changes of lipid peroxidation in donor lung tissue after lung reperfusion in donor dogs, and to investigate the changes of apoptotic gene expression in donor canine lung implantation after ischemic treatment. [methods] 12 dogs were randomly selected.Donor recipients were formed and allogeneic left single lung transplantation was performed.Two groups were randomly divided into two groups: the control group (control group): 6 pairs of dogs were transplanted with single donor donor allograft on the left side without the intervention of ischemic postprocessing.In the ischemic post-treatment group, the donor donor allogeneic single lung transplantation was performed on the left side of the dog. After the donor lung was implanted in the conventional way, 10 s reperfusion and 10 s reperfusion were performed at the early stage of reperfusion, and the total duration was 1 min after ischemia.The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in donor lung samples were detected at 1h and 2h after lung reperfusion, and the expression of apoptotic gene Bcl-2 (Bax) was detected by RT-PCR technique at 12 h after lung reperfusion.The pathological changes of lung tissue were observed under light microscope at 1h and 2 h after lung implantation in donor dogs. SPSS 13.0 statistical software was used to deal with the pathological changes. [results] the mean implantation time of donor lung was 35.9 卤1.7 minutes.The level of SOD activity in the donor lung tissue in the ischemic group was significantly lower than that in the control group at 1 h and 2 h after ischemia compared with that in the control group (P 0.05), and that in the control group was higher than that in the experimental group.The expression of Bc12 protein in lung tissue was up-regulated at 1h after reperfusion, while the expression of Bax protein was down-regulated (P 0.05), and the inflammatory response of lung tissue in ischemic group was slightly different from that in control group under light microscope. [conclusion]Post-treatment can inhibit the accumulation of oxidized free radicals during reperfusion.By inhibiting the expression of pro-apoptotic Bax protein and promoting the expression of anti-apoptotic Bc12 protein, the endogenous apoptotic pathway could be affected by post-ischemic treatment.
【作者单位】：中山大学附属第一医院胸外科;
【基金】：广东省自然科学基金博士启动项目(9451008901002331)
【分类号】：R363

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