电针对动脉粥样硬化模型家兔血清中CD40、CD40L影响的实验研究

发布时间：2018-04-24 02:07

本文选题：动脉粥样硬化 + CD40　；参考：《辽宁中医药大学》2017年硕士论文

【摘要】：目的:观察电针内关、关元、足三里穴对动脉粥样硬化模型家兔的干预作用,通过检测各组血清中CD40、CD40L的表达水平,探讨电针内关、关元、足三里穴抑制动脉粥样硬化模型家兔炎症反应的作用机制。材料与方法:应用26只健康雄性新西兰雄性家兔(每只2-2.5kg,2-3月龄),适应性喂养一周后,随机分为正常组和造模组,其中正常组7只,造模组19只。正常组给予普通饲料喂养,造模组给予高脂饲料和普通饲料混合喂养,高脂喂养4周后对造模组行颈动脉球囊损伤术。高脂喂养8周后,从正常组和造模组中各随机选取1只家兔检测造模是否成功,采用HE染色的检测方法观察家兔颈动脉粥样硬化斑块病理形态学变化。将造模组(18只)随机分为三组(每组n=6):模型组、电针组、西药组。电针组选取内关(双侧)、足三里(双侧)、关元穴进行治疗,每日1次;西药组给予阿托伐他汀钙片悬浊液1mg/kg/d灌胃给药。6天为1疗程,疗程间休息1日,共4个疗程。第四个疗程治疗结束后对所有家兔进行处死,耳缘经脉采血,分离血清,取颈动脉固定于4%多聚甲醛中48小时后HE染色,分析颈动脉的病理学改变,采用酶联免疫吸附测定法对血清中CD40、CD40L进行分析检测。结果:1.电针内关、关元、足三里穴对血清中CD40的影响:与正常组进行比较,模型组、电针组、西药组血清中CD40的表达增高,差异具有统计学意义(P0.05);与模型组比较,电针组和西药组家兔血清中CD40表达水平降低,差异有统计学意义(P0.05);与西药组比较,电针组血浆CD40水平无明显差异,差异无统计学意义(P0.05)。2.电针内关、关元、足三里穴对血清中CD40L的影响:与正常组进行比较,模型组、电针组、西药组血清中CD40L的表达增高,差异具有统计学意义(P0.05);与模型组比较,电针组和西药组家兔血清中CD40L水平降低,差异有统计学意义(P0.05);与西药组比较,电针组血CD40L水平无明显差异,差异无统计学意义(P0.05)。结论:1.降低血清中CD40的浓度,对动脉粥样硬化模型家兔血清中炎症反应起到抑制作用,体现了应用电针刺激内关、关元、足三里穴位的通络、活血、化痰功效对动脉粥样硬化的预防及治疗可能具有积极的意义。2.降低血清中CD40L浓度,对动脉粥样硬化模型家兔血清中炎症反应起到抑制作用,体现了应用电针刺激内关、关元、足三里穴位的通络、活血、化痰功效对动脉粥样硬化的预防及治疗可能具有积极的意义。
[Abstract]:Objective: to observe the intervention effect of electroacupuncture at Neiguan, Guanyuan and Zusanli on atherosclerosis model rabbits, and to investigate the expression of CD40T CD40L in serum of each group, and to explore the effect of electroacupuncture on Neiguan and Guanyuan. The mechanism of Zusanli (Zusanli) on inhibiting inflammatory response in atherosclerotic rabbits. Materials and methods: Twenty-six healthy male New Zealand rabbits (2-2.5kg / 2 ~ (-3) months old) were randomly divided into normal group (n = 7) and model group (n = 19) after a week of adaptive feeding. The normal group was fed with normal diet, the model group was fed with high fat diet and common feed, and the model group was treated with carotid artery balloon injury after 4 weeks of high fat feeding. After 8 weeks of high fat feeding, one rabbit was randomly selected from the normal group and the model group to detect whether the model was successful or not, and the pathological morphology of carotid atherosclerotic plaque was observed by HE staining. The model group (n = 18) was randomly divided into three groups: model group, electroacupuncture group and western medicine group. In the electroacupuncture group, Neiguan (bilateral and Zusanli) was treated once a day, and the western medicine group was treated with 1mg/kg/d, which was administered by oral administration of Atto calcareous tablets for 6 days, with 1 course of rest between the two courses of treatment for 4 courses. At the end of the fourth course of treatment, all rabbits were killed. Blood was collected from the meridians of the ear margin, serum was isolated, and the carotid artery was fixed in 4% paraformaldehyde for 48 hours. The pathological changes of the carotid artery were analyzed. Enzyme linked immunosorbent assay (Elisa) was used to detect CD40 C 40 L in serum. The result is 1: 1. The effect of electroacupuncture at Neiguan, Guanyuan and Zusanli on CD40 in serum: compared with normal group, the expression of CD40 in serum of model group, electroacupuncture group and western medicine group was higher than that of model group, and the difference was statistically significant (P 0.05). Compared with the western medicine group, there was no significant difference in the plasma CD40 level between the electroacupuncture group and the western medicine group, but there was no significant difference between the electroacupuncture group and the western medicine group. The effect of electroacupuncture at Neiguan, Guanyuan and Zusanli on CD40L in serum: compared with normal group, the expression of CD40L in serum of model group, electroacupuncture group and western medicine group was higher than that of model group, and the difference was statistically significant (P 0.05). The serum CD40L level in the electroacupuncture group and the western medicine group was lower than that in the western medicine group (P 0.05), but there was no significant difference in the serum CD40L level between the electroacupuncture group and the western medicine group (P 0.05). Conclusion 1. Lowering the concentration of CD40 in serum can inhibit the inflammatory reaction in the serum of atherosclerotic model rabbits. It reflects the application of electroacupuncture to stimulate Neiguan, Guanyuan, Zusanli acupoints to clear the collaterals and promote blood circulation. Resolving phlegm effect may have positive significance to the prevention and treatment of atherosclerosis. Lowering the concentration of CD40L in serum can inhibit the inflammatory reaction in the serum of atherosclerosis model rabbits. It reflects the stimulation of acupoints of Neiguan, Guanyuan and Zusanli by electroacupuncture, which can activate the blood circulation and the collaterals of the acupoints of Guanyuan, Guanyuan and Zusanli. The effect of resolving phlegm may be of positive significance to the prevention and treatment of atherosclerosis.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245;R-332

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