细粒棘球蚴囊液对体外培养小鼠T淋巴细胞影响的初步研究

发布时间：2018-04-28 14:35

本文选题：囊液 + T细胞亚群　；参考：《中国免疫学杂志》2013年03期

【摘要】：目的:观察细粒棘球蚴囊液对体外培养的BABL/c小鼠T淋巴细胞的影响。方法:BABL/c小鼠脾细胞与100μl、200μl、300μl、500μl不同浓度的细粒棘球蚴囊液共培养,流式细胞仪检测CD3+CD4+/CD3+CD8+T淋巴细胞亚群和CD4+CD25+T细胞,RT-PCR法检测Foxp3基因的表达量;相同浓度的囊液和BABL/c小鼠脾细胞悬液共培养,在0、12、24、36、48小时收集细胞,检测T淋巴细胞亚群和Foxp3基因表达情况。结果:随着囊液浓度的增加,CD4+T细胞比例逐渐下降,CD8+T细胞比例上升,CD4+/CD8+的比值下降;CD4+CD25+T细胞则没有随着囊液量的增加而上升,而是在100μl组达到高峰值。RT-PCR的结果显示,在低浓度100μl和200μl组的时候Foxp3 mRNA的相对表达量最高。结论:细粒棘球蚴囊液促使CD4+/CD8+的比值下降,Treg细胞增加,可能在包虫免疫逃逸过程中发挥一定作用。
[Abstract]:Aim: to observe the effect of Echinococcus granulosus capsule fluid on T lymphocytes of BABL/c mice in vitro. Methods the spleen cells of 1 / 1 Babel / c mice were co-cultured with 100 渭 l / 200 渭 l / 300 渭 l / 500 渭 l Echinococcus granulosus sac fluid. CD3 CD4 / CD3 CD8 T lymphocyte subsets and CD4 CD25 T cells were detected by flow cytometry and the expression of Foxp3 gene was detected by reverse transcription-polymerase chain reaction (RT-PCR). The same concentration of sac fluid and spleen cell suspension of BABL/c mice were co-cultured. The cells were collected for 48 hours at 012A 2436 and the expression of T lymphocyte subsets and Foxp3 gene were detected. Results: with the increase of sac fluid concentration, the proportion of CD 4 T cells decreased gradually. The ratio of CD 4 / CD 8 increased. The ratio of CD 4 / CD 8 decreased. The ratio of CD 4 CD25 T cells did not increase with the increase of the volume of sac fluid, but reached a high peak value in 100 渭 l group. The relative expression of Foxp3 mRNA was the highest in 100 渭 l and 200 渭 l groups. Conclusion: Echinococcus granulosus sac fluid can decrease the ratio of CD4 / CD8 and increase Treg cells, which may play a role in the immune escape of hydatid.
【作者单位】：石河子大学医学院免疫学教研室新疆地方病与民族高发病重点实验室;石河子大学第一附属医院肝胆外科;
【基金】：国家自然科学基金(81060227) 兵团科技支疆项目(2011AB034)
【分类号】：R392.4

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