AMPA受体及其GluR2亚基在大鼠初级视皮层可塑性中的作用研究

发布时间：2018-05-09 03:27

本文选题：初级视皮层 + 视觉诱发电位　；参考：《中国现代医学杂志》2013年28期

【摘要】：目的观察闪光刺激对大鼠初级视皮层诱发电位的影响,探讨AMPA受体及其GluR2亚基在大鼠初级视皮层可塑性中的作用。方法 24只成年SD大鼠随机分为3组:A组(空白对照组:不接受闪光刺激)、B组(光刺激组:接受紫光闪光刺激)、C组(阻断剂组:接受紫光闪光刺激并注射AMPA受体阻断剂)。各组大鼠麻醉、固定后分别在初级视皮层(V1区)植入记录电极,在额叶植入参考电极,在V1区(与记录电极同侧)植入微细玻璃微电极做微量注射,B组和C组手术恢复后连续5 d每天于固定时间行紫光闪光刺激1 h,之后行微量注射生理盐水和AMPA受体阻断剂后,在相同闪光刺激条件下,给予一串高频刺激,记录高频刺激前后初级视皮层(V1区)的视觉诱发电位,分析两组视觉诱发电位的幅值和潜伏期的变化;western blot检测各组大鼠强直性闪光刺激前后初级视皮层GluR2蛋白含量的变化。结果 B组和C组在接受强直性闪光刺激后诱发电位幅值较之前明显升高,且潜伏期变短(均P0.01),在强直性闪光刺激后,C组幅值显著高于B组、潜伏期显著短于B组(均P0.01);在强直性闪光刺激之前,B组和C组的幅值和潜伏期均差异无显著性。western blot结果显示,B组大鼠GluR2含量在强直性闪光刺激前显著高于强直性闪光刺激后,但显著低于A组(均P0.05),C组GluR2含量与B组在强直性闪光刺激前后均差异无显著性。结论强直性闪光刺激能使大鼠初级视皮层视觉诱发电位呈现LTP样反应,AMPA及其GluR2亚基参与了闪光刺激致大鼠初级视皮层的可塑性变化。
[Abstract]:Objective to observe the effect of flash stimulation on the primary visual cortex evoked potential (VEP) in rats and to explore the role of AMPA receptor and its GluR2 subunit in the plasticity of primary visual cortex in rats. Methods Twenty-four adult Sprague-Dawley rats were randomly divided into 3 groups: control group: no flash stimulation group B (light stimulation group: group C received purple light flash stimulation) (blocker group: received purple light flash stimulation and injected AMPA receptor blocker). Rats in each group were anesthetized. After fixation, recording electrodes were implanted in the primary visual cortex (V1) and reference electrodes were implanted in the frontal lobe. The microglass-microelectrode was implanted in the V1 region (the same side as the recording electrode) for 5 days after the recovery of the operation in group B and C, followed by microinjection of physiological saline and AMPA receptor blocker for 1 hour at a fixed time. Under the same flash stimulation condition, a series of high frequency stimuli were given to record the visual evoked potentials of the V1 region of the primary visual cortex before and after the high frequency stimulation. The changes of amplitude and latency of visual evoked potential (VEP) in two groups were analyzed. The changes of GluR2 protein content in primary visual cortex were detected by western blot before and after ankylosing flash stimulation. Results the amplitude of evoked potential of group B and group C was significantly higher than that of group B (P 0.01), and the amplitude of group C was significantly higher than that of group B (P < 0.01), and the amplitude of group C was significantly higher than that of group B (P < 0.01). The results of western blot showed that the content of GluR2 in group B was significantly higher than that in group B (P < 0.01), but there was no significant difference in amplitude and latency between group B and group C before and after flash stimulation. But the content of GluR2 in group C was significantly lower than that in group A (P 0.05) and there was no significant difference between group B and group B before and after the stimulation. Conclusion the visual evoked potentials of the primary visual cortex of the rats presented LTP like response. AMPA and its GluR2 subunits were involved in the plasticity of the primary visual cortex induced by the flash stimulation.
【作者单位】：漯河医学高等专科学校生理学教研室;漯河医学高等专科学校预防医学教研室;
【基金】：漯河医学高等专科学校2012校级自然科学研究资助项目(No:2010S07)
【分类号】：R339.1

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