小鼠脑室下区细胞增殖能力随年龄增加逐渐下降与β-catenin的相关研究

发布时间：2018-05-20 02:28

本文选题：脑室下区 + 前体细胞增殖　；参考：《神经解剖学杂志》2013年03期

【摘要】：目的:脑内成体干细胞状态和年龄的关系非常密切,本研究探讨小鼠老化过程中脑室下区(subven-tricular zone,SVZ)内成体干细胞随年龄增加增殖活性的变化和脑内β-catenin信号的相关性。方法:C57B/L小鼠分别饲养至2,12,18月时处死,各组动物处死前腹腔注射BrdU(60 mg/kg,处死前3 d开始注射,连续注射3 d,1次/d)。部分动物断头取脑,冰台上游离前额皮层,冷冻备用,部分动物灌注固定,连续冠状冰冻切片备用。利用免疫组织化学方法检测SVZ区BrdU和Ki67表达。利用Western blot技术检测前额皮层内β-catenin的表达水平。结果:12,18月时小鼠SVZ区BrdU和Ki67阳性细胞的数量与2月时比较明显有所减少(P0.05)。Western blot结果可见前额皮层内β-catenin的表达水平在2月时达高峰,12月和18月时逐渐减少。结论:SVZ区干细胞的增殖能力随年龄增加逐渐下降可能与脑内β-catenin信号的减弱有关。
[Abstract]:Aim: the relationship between the state of adult stem cells in brain and age is very close. In this study, we investigated the relationship between the proliferative activity of adult stem cells in subventricular area of subventricular region and the signal of 尾 -catenin during aging in mice. Methods C57B / L mice were fed until 2o 12 and killed at 18 months. BrdU(60 mg / kg was injected intraperitoneally 3 days before death. Some animals were decapitated, the prefrontal cortex was free on the ice table, frozen, and some animals were infused and fixed, and continuous coronal frozen sections were used. The expression of BrdU and Ki67 in SVZ was detected by immunohistochemical method. The expression of 尾-catenin in prefrontal cortex was detected by Western blot technique. Results the expression of 尾 -catenin in the prefrontal cortex reached its peak in February and decreased gradually in December and 18 months. The results showed that the number of BrdU and Ki67 positive cells in the SVZ region of mice at 12 and 18 months was significantly lower than that in the second month. Western blot showed that the expression of 尾 -catenin in the prefrontal cortex reached its peak in February and decreased gradually in December and 18 months. Conclusion the ability of stem cells to proliferate gradually decreases with the increase of age, which may be related to the decrease of 尾 -catenin signal in brain.
【作者单位】：兰州大学基础医学院人体解剖与组织胚胎学研究所;甘肃省灵台县什字中心卫生院;兰州大学基础医学院生物化学与分子生物学研究所;
【基金】：国家自然科学基金(30872731) 甘肃省自然科学基金项目(1010RJZA092)
【分类号】：R338

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