阿司匹林对人脐血晚期内皮祖细胞功能的影响

发布时间：2018-05-20 06:37

本文选题：阿司匹林 + 内皮祖细胞　；参考：《中国实验血液学杂志》2013年04期

【摘要】：本研究旨在观察阿司匹林对人脐血晚期内皮祖细胞(endothelial progenitor cells,EPC)功能的影响。采用密度梯度离心法及免疫磁珠分选法从脐血获得CD34+的单个核细胞,将其接种在人纤维连接蛋白包被的培养板上,培养2周后收集贴壁细胞,通过流式细胞术、免疫荧光、RT-PCR、DiI-Ac-LDL的摄取及体外血管形成能力等方法对其进行鉴定。用不同浓度阿司匹林(终浓度分别为0.1,1,10,100,1 000,10 000μmol/L)处理EPC 24 h,然后分别采用CCK-8比色法、Transwell小室、黏附能力测定实验来观察EPC的增殖能力、迁移能力和黏附能力的变化。结果表明:低浓度的阿司匹林(0.1-1 000μmol/L)对脐血晚期EPC的增殖无明显影响,但可增强晚期EPC的黏附及迁移能力,而高浓度的阿司匹林(10 000μmol/L)显著抑制晚期EPC的增殖和迁移,但对其黏附能力无明显影响。结论:低浓度的阿司匹林可增强晚期EPC的黏附及迁移能力,而高浓度的阿司匹林抑制晚期EPC的增殖、黏附及迁移能力,从而抑制内皮细胞的生成。
[Abstract]:The purpose of this study was to observe the effect of aspirin on the function of endothelial progenitor cells (EPC) in human umbilical cord blood. The density gradient centrifugation and immunomagnetic beads were used to obtain the mononuclear cells from the umbilical cord blood from the umbilical cord blood. It was inoculated on the culture plate of the human fibronectin envelope and collected the adherent cells after 2 weeks. It was identified by flow cytometry, immunofluorescence, RT-PCR, DiI-Ac-LDL uptake and angiogenesis in vitro. EPC 24 h was treated with different concentrations of aspirin (the final concentration was 0.1,1,10100,1 000,10 000 mu mol/L). Then CCK-8 specific colorimetry, Transwell chamber and adhesion assay were used to observe the increase of EPC. The results showed that low concentration of aspirin (0.1-1 000 mol/L) had no obvious effect on the proliferation of advanced EPC in umbilical cord blood, but enhanced the adhesion and migration of advanced EPC, while high concentration of aspirin (10000 mu mol/L) significantly inhibited the proliferation and migration of late EPC, but had no adhesion to the late EPC. Conclusion: low concentration of aspirin can enhance the adhesion and migration of advanced EPC, while high concentration of aspirin inhibits the proliferation, adhesion and migration of advanced EPC, thus inhibiting the formation of endothelial cells.
【作者单位】：苏州大学附属第一医院江苏省血液研究所;卫生部血栓与止血重点实验室;苏州大学附属第二医院骨外科;苏州大学唐仲英血液学研究中心;首都医科大学附属北京朝阳医院肿瘤科;
【基金】：国家自然科学基金资助项目(面上项目)(项目批准号:81070415)
【分类号】：R363

【相似文献】