诱导金属硫蛋白表达对大鼠缺血再灌注心肌细胞凋亡及核因子κB表达的影响

发布时间：2018-05-28 06:13

本文选题：缺血再灌注 + 金属硫蛋白　；参考：《中国老年学杂志》2013年13期

【摘要】：目的探讨金属硫蛋白(MT)对大鼠缺血再灌注(I/R)心肌细胞凋亡及核因子κB(NF-κB)表达水平的影响。方法 32只SD大鼠随机分成实验组、对照组,每组16只。分别予硫酸锌(ZnSO4)和蒸馏水预处理。预处理后构建Langendorff离体心脏I/R动物模型,动态观测缺血前及再灌注期左心室发展压(LVDP)、左心室压力最大上升和下降速率(±dp/dtmax)、冠状动脉流出量(CF);测定冠状动脉流出液肌酸磷酸激酶MB同工酶(CK-MB)的漏出率;Western印迹法检测金属硫蛋白(MT)表达;TUNEL法检测心肌细胞凋亡;免疫组化法检测NF-κB的活化水平。结果与对照组比较,实验组再灌注期LVDP、±dp/dtmax及CF得到改善(P0.05);CK-MB的漏出率减少;金属硫蛋白的表达增加;细胞凋亡指数及NF-κB表达减少(均P0.01)。结论 MT下调NF-κB表达可能与其抑制I/R心肌细胞凋亡有关。
[Abstract]:Objective to investigate the effects of metallothionein (MTT) on myocardial apoptosis and the expression of nuclear factor 魏 B (NF- 魏 B) NF- 魏 B in ischemic reperfusion (I / R) rat cardiomyocytes. Methods 32 SD rats were randomly divided into experimental group (n = 16) and control group (n = 16). Zinc sulfate (ZnSO _ 4) and distilled water were pretreated respectively. I / R animal model of Langendorff isolated heart was constructed after preconditioning. Dynamic observation of left ventricular pressure before ischemia and reperfusion, left ventricular pressure maximum rise and fall rate (卤DP / dtmax1), coronary outflow volume (CFN). The leakage rate of creatine phosphokinase MB isoenzyme CK-MBin coronary effluents was determined by Western blotting The expression of metallothionein (MTT) was detected by Tunel method. The activation level of NF- 魏 B was detected by immunohistochemistry. Results compared with the control group, LVDP, 卤dp/dtmax and CF improved the leakage rate of CK-MB, increased the expression of metallothionein, and decreased the apoptosis index and NF- 魏 B expression in the experimental group during reperfusion (all P 0.01). Conclusion the down-regulation of NF- 魏 B expression by MT may be related to the inhibition of I / R cardiomyocyte apoptosis.
【作者单位】：贵阳医学院附属医院心脏内科;
【基金】：贵州省科学技术基金〔黔科合J字(2007)2099〕
【分类号】：R363

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