鼻窦炎口服液对急性鼻-鼻窦炎模型大鼠鼻黏膜EGF及EGFR表达的影响

发布时间：2018-05-28 15:29

本文选题：鼻窦炎口服液 + 鼻渊　；参考：《成都中医药大学》2015年博士论文

【摘要】：目的：通过大鼠急性鼻-鼻窦炎动物模型进行实验,观察鼻窦炎口服液对急性鼻-鼻窦大鼠鼻黏膜表皮生长因子及其受体的影响,进一步探讨其治疗鼻渊的机制,结合导师学术思想更好地指导临床用药。方法：60只SD大鼠随机分为①空白组；②模型组：③鼻窦炎口服液低剂量组；④鼻窦炎口服液中剂量组；⑤鼻窦炎口服液高剂量组；⑥阿莫西林克／拉维酸钾组。急性鼻-鼻窦炎模型依据叶菁报道的方法改良造模。造模7天后,空白组和模型组按5ml·kg-1·d-1灌服无菌水,其余各组分别灌服相应的药物。从造模第1天开始,观察大鼠一般情况变化(体温、体重、局部症状、鼻分泌物等)。用药7天后每组随机选取6只大鼠,测得鼻分泌物PH值；取外周血测得白细胞(WBC)计数与中性粒细胞百分比(N)。之后立即处死大鼠,取得鼻粘膜标本,用免疫组化SP法检测各组大鼠鼻黏膜EGF和EGFR；用RT-PCR法测定大鼠鼻粘膜EGFmRNA的表达：在光学显微镜下观察大鼠鼻黏膜的病理形态变化。结果：1.模型组大鼠鼻腔分泌物pH值与空白组相比,显著性升高(P0.01)。鼻窦炎口服液低剂量组pH值与空白组相比升高,差异有统计学意义(P0.05)。各治疗组药物均能显著降低鼻腔分泌物pH值(P0.01),其中以鼻窦炎口服液高剂量组、阿莫西林/克拉维酸钾组最为明显。2.急性鼻-鼻窦炎大鼠WBC计数及N%较空白组明显升高(P0.01),证明本次实验造模是成功的；各治疗组药物均可使升高的WBC计数下降,其中,鼻窦炎口服药液高剂量组,阿莫西林/克拉维酸钾组效果最明显(P0.01)。3.与空白组比较,模型组大鼠鼻粘膜组织EGF免疫组织化学检测积分光密度(MD)显著降低(P0.05)。与模型组相比,西药组、高剂量组和中剂量组组大鼠鼻粘膜组织EGF免疫组织化学检测积分光密度(MD)均有显著升高(P0.05),低剂量组有升高。4.与空白组比较,模型组大鼠鼻粘膜组织EGFR免疫组织化学检测积分光密度(MD)极显著降低(P0.01),提示造模成功。与模型组相比,西药组、高剂量组和中剂量组大鼠鼻粘膜组织EGFR免疫组织化学检测积分光密度(MD)均有极显著升高(P0.01),低剂量组有升高。5.与空白组比较,模型组大鼠鼻粘膜组织EGF基因的表达有极显著降低(P0.01)。与模型组比较,西药组、高剂量组大鼠鼻粘膜组织EGF基因的表达有极显著升高(P0.01)；中剂量组大鼠鼻粘膜组织EGF基因的表达有显著升高(P0.05)。6.光镜观察显示：模型组大鼠鼻粘膜可见明显的急性炎性病理改变,用药7天后,除鼻窦炎口服液低剂量组,各治疗组药物均使炎性改变减轻,以鼻窦炎口服液高、阿莫西林/克拉维酸钾组效果最明显。结论：1.鼻窦炎口服液可以降低急性鼻-鼻窦炎大鼠升高的鼻分泌物pH值至正常范围。2.各组大鼠鼻黏膜EGF的MOD值间有显著性差异(P0.05),EGF在大鼠鼻黏膜中呈固有表达。西药组、鼻窦炎口服液高、中剂量组可增强ARS大鼠鼻黏膜EGF基因的表达,发挥修复作用,有效促进鼻窦黏膜创面愈合。3.各组大鼠鼻黏膜EGFR的MOD值间有极显著差异(P0.01),EGFR在大鼠鼻黏膜中呈固有表达。EGFR可在炎症条件下被诱导。鼻窦炎口服液高,中剂量组、阿莫西林/克拉维酸钾组能有效的控制局部炎症,加强鼻黏膜组织修复和功能恢复。4.鼻窦炎口服液能有效减轻急性鼻-鼻窦炎大鼠局部及全身的炎性改变,其中阿莫西林/克拉维酸钾组和鼻窦炎口服液高剂量组效果最为明显。
[Abstract]:Objective : To observe the effect of nasal sinusitis oral liquid on epidermal growth factor and its receptor in nasal mucosa of rats with acute nasal - nasal sinuses by using animal model of acute nasal - sinusitis in rats .
( 2 ) model group : ( 3 ) low - dose group of nasal sinusitis oral liquid ;
( 4 ) the dosage group in nasal sinusitis oral liquid ;
( 5 ) High - dose group of nasal sinusitis oral liquid ;
6 . The model of acute nose - sinusitis was improved by the method of Cyanine . After 7 days of molding , the blank group and the model group were administered with sterile water according to 5 ml 路 kg -1 路 d -1 , and the rest groups were respectively filled with the corresponding drugs . From the first day of the molding , the general conditions of the rats were observed ( temperature , body weight , local symptoms , nasal discharge , etc . ) . 6 rats were randomly selected from each group after 7 days of administration , and the PH value of nasal secretion was measured .
The white blood cell ( WBC ) count and neutrophil percentage ( N ) were measured in peripheral blood . Then the rats were sacrificed immediately , the nasal mucosa specimens were obtained , and EGF and EGFR in nasal mucosa of each group were detected by immunohistochemistry SP method .
The expression of EGFmRNA in nasal mucosa of rats was measured by RT - PCR . The results showed that : 1 . The pH value of nasal secretion was significantly higher than that in blank group ( P0.01 ) .
Compared with the model group , the expression of EGF gene in nasal mucosa of model group was significantly decreased ( P0.01 ) . Compared with the model group , the expression of EGF gene in nasal mucosa of model group was significantly decreased ( P0.01 ) . Compared with the model group , the expression of EGF gene in nasal mucosa of model group was significantly increased ( P0.01 ) .
The expression of EGF gene in nasal mucosa of rat nasal mucosa was significantly increased ( P0.05 ) .
【学位授予单位】：成都中医药大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：R285.5;R-332

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