褪黑素对动脉粥样硬化模型兔血脂、TLR4表达、炎症因子水平和斑块进展的影响

发布时间：2018-06-03 09:52

本文选题：褪黑素 + 动脉粥样硬化　；参考：《安徽医科大学学报》2013年09期

【摘要】：目的探讨褪黑素(MT)对动脉粥样硬化(AS)模型兔血脂、主动脉Toll样受体4(TLR4)蛋白表达、血炎症因子水平和AS进展的影响。方法复制实验性兔AS模型。36只新西兰大白兔,随机均分为3组。正常组:给予普通饲料喂养;模型组:给予高胆固醇饲料喂养;MT组:给予高胆固醇饲料加MT喂养。12周后分别检测血清血脂[总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、三酰甘油(TG)]水平和血清炎症因子[肿瘤坏死因子α(TNF-α)、高敏C反应蛋白(hs-CRP)]水平,观察主动脉病理形态学变化,免疫组织化学法和Western blot法检测兔主动脉TLR4蛋白水平。结果 12周高脂喂养成功建立实验性兔AS模型;与正常组相比,模型组TC、LDL-C、HDL-C、TG、TNF-α、hs-CRP明显升高,内膜厚度与内膜/中膜厚度比明显升高,动脉TLR4蛋白表达较正常组显著升高(P0.05)。与模型组相比,MT组血清TC、LDL-C、TG、TNF-α、hs-CRP明显下降,HDL-C明显升高,主动脉中TLR4表达减少,内膜厚度、内膜/中膜厚度比明显降低(P0.05)。结论MT可能通过改善血脂、降低动脉TLR4蛋白表达和血炎症因子水平抑制实验性兔AS进展。
[Abstract]:Objective to investigate the effects of melatonin (MT) on the expression of serum lipids, aortic Toll like receptor 4 (TLR4) protein, the level of serum inflammatory factors and the progression of as in atherosclerotic rabbits. Methods 36 New Zealand white rabbits were randomly divided into 3 groups. Normal group: fed with common feed; Model group: high cholesterol diet feeding MT group: high cholesterol diet plus MT feeding. 12 weeks after feeding, serum lipids were measured respectively [total cholesterol TCU, low density lipoprotein cholesterol (LDL-CU), high density lipoprotein cholesterol (HDL-CU), triacylglycerol (triacylglycerol)]. The levels of serum inflammatory cytokines (TNF- 伪 TNF- 伪, Gao Min C-reactive protein hs-CRP), The histopathologic changes of aorta were observed and the level of TLR4 protein in rabbit aorta was detected by immunohistochemical method and Western blot method. Results the experimental as model was successfully established by high-fat feeding at the 12th week, and compared with the normal group, the TCLDL-CnHDL-CnTG-TNF- 伪 TNF- 伪 hs-CRP, the ratio of intimal thickness to intimal / media thickness, and the expression of TLR4 protein were significantly increased in the model group compared with the normal group (P 0.05). Compared with the model group, the serum TCU LDL-C and TNF- 伪 hs-CRP in the MT group were significantly decreased HDL-C, TLR4 expression, intimal thickness and intimal / media thickness ratio were significantly decreased (P 0.05). Conclusion MT may inhibit the development of experimental rabbit as by improving blood lipids, decreasing the expression of arterial TLR4 protein and the level of blood inflammatory factors.
【作者单位】：安徽医科大学第一附属医院心血管内科;安徽医科大学分子生物学实验室;
【基金】：安徽省自然科学基金(编号:11040606Q20、11040606M157) 安徽省高等学校省级自然科学研究项目(编号:9021285201) 高等学校博士学科点专项科研基金(编号:2012342012005)
【分类号】：R543.5;R-332

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