耐碳青霉烯类弗氏柠檬酸杆菌的表型及基因型特征研究

发布时间：2018-06-03 12:36

本文选题：药物敏感性试验 + 碳青霉烯类　；参考：《中华医院感染学杂志》2013年24期

【摘要】：目的分析耐碳青霉烯类弗氏柠檬酸杆菌的表型和基因型特征,为临床资料提供参考依据。方法收集2008年1月-2011年12月临床分离的18株非重复耐碳青霉烯类弗氏柠檬酸杆菌;采用琼脂稀释法检测弗氏柠檬酸杆菌对抗菌药物的敏感性;PCR扩增检测β-内酰胺酶基因;质粒转移接合试验检测耐药β-内酰胺酶耐药基因的可传递性。结果替加环素、米诺环素和多西环素对耐碳青霉烯类弗氏柠檬酸杆菌有较好的抗菌活性,MIC50值分别为1、4、4μg/ml,MIC90值分别1、8、4μg/ml,弗氏柠檬酸杆菌对常用抗菌药物表现为高度耐药,MIC90多数≥128μg/ml;PCR检测结果显示,所有菌株均产生KPC-2型基因碳青霉烯酶;除一株细菌产CTX-M-15型基因ESBLs外,其余菌株均产CTX-M-14型基因ESBLs;所有菌株均产CMY-2型基因质粒AmpC酶;质粒转移接合试验结果表明,携带KPC-2型基因碳青霉烯酶耐药基因的质粒可从供体菌转移至敏感细胞中。结论临床分离的耐碳青霉烯类弗氏柠檬酸杆菌呈泛耐药特征,使临床抗感染治疗的难度增大,临床应注意预防控制此类耐药菌株引起的进一步流行传播。
[Abstract]:Objective to analyze the phenotypic and genotypic characteristics of carbapene-resistant Streptococcus flexneri and to provide reference for clinical data. Methods from January 2008 to December 2011, 18 strains of non-repeated carbapenem resistant citric acid bacilli were collected, and 尾 -lactamase gene was detected by Agar dilution assay. The transferability of 尾-lactamase resistance gene was detected by plasmid transfer conjugation test. Results tigicycline, The MIC50 values of minocycline and doxycycline against carbon-penicillin-resistant citric acid bacilli were 1 ~ 4 渭 g / ml, respectively, and the MIC90 values were 1 ~ 8 渭 g / ml, respectively. The results of PCR analysis showed that most of the common antimicrobial agents were highly resistant to MIC90 or more than 128 渭 g 路ml ~ (-1) 路ml ~ (-1). All strains produced carbapenase of KPC-2 type gene, except one strain that produced CTX-M-15 type gene ESBLs, all other strains produced CTX-M-14 type gene AmpC enzyme, all strains produced CMY-2 type gene plasmid AmpC enzyme, and the result of plasmid transfer conjugation test showed that, Plasmids carrying carbapenem resistance gene of KPC-2 gene can be transferred from donor bacteria to sensitive cells. Conclusion the clinical isolates of carbapenem resistant citric acid bacilli have the characteristics of pan-resistance, which makes it more difficult for clinical anti-infective treatment. Clinical attention should be paid to prevent and control the further epidemic spread caused by this kind of resistant strains.
【作者单位】：丽水市人民医院检验科;
【基金】：浙江省自然科学基金项目(LY12H19003)
【分类号】：R378

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