肠分化细胞黏蛋白O-型糖链合成抑制导致MUC2表达减低以及对细菌侵袭易感

发布时间：2018-06-14 15:29

  本文选题：O-型糖链 + benzyl-α-GalNAc　； 参考：《第三军医大学学报》2013年19期

【摘要】：目的探讨O-型糖链合成的抑制对肠分化细胞内细菌侵袭数量和细胞内MUC2表达水平的影响。方法对肠分化细胞(HT-29-Gal)用O-型糖链抑制剂(benzyl-N-acetyl-α-D-galactosaminide,benzyl-α-GalNAc)处理,采用Real-time PCR和Western blot检测MUC2 mRNA和蛋白的表达情况。并将HT-29-Gal细胞及benzyl-α-GalNAc处理的HT-29-Gal细胞分别与肠致病性大肠杆菌(enteropathogenic E.coli,EPEC)和肠出血性大肠杆菌(EHEC O157∶H7)37℃孵育2 h,再加入100μg/mL的庆大霉素,杀灭细胞外及粘附于细胞表面的细菌。最后采用系列稀释克隆计数法观察benzyl-α-GalNAc处理的HT-29-Gal细胞对细菌侵袭的影响。结果 Real-time PCR和Western blot检测发现经benzyl-α-GalNAc处理的HT-29-Gal细胞MUC2的mRNA和蛋白表达水平明显降低(P0.05)。侵袭入benzyl-α-GalNAc处理的HT-29-Gal细胞的EPEC和EHEC O157∶H7的数量较对照细胞显著增加(P0.05)。结论抑制HT-29-Gal细胞黏蛋白O-型糖链的合成导致侵袭入细胞内细菌数量增加和MUC2的表达降低。
[Abstract]:Objective to investigate the effect of the inhibition of O-type sugar chain synthesis on the number of bacterial invasion and the expression of MUC2 in intestinal differentiation cells. Methods the intestinal differentiation cells were treated with benzyl-N-acetyl- 伪 -D-galactosaminide benzyl- 伪 -GalNAc. real-time PCR and Western blot were used to detect the expression of MUC2 mRNA and protein. HT-29-Gal cells and benzyl- 伪 -GalNAc treated HT-29-Gal cells were incubated with enteropathogenic E.coli EPECC and EHEC O157: H737 鈩，

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