大鼠坐骨神经慢性压迫后脊髓背角GCH1基因表达与疼痛的关系

发布时间：2018-06-17 02:23

本文选题：神经病理性疼痛 + GCH基因　；参考：《中国疼痛医学杂志》2013年04期

【摘要】：目的:探讨大鼠坐骨神经慢性压迫(chronic constriction injury,CCI)后,脊髓背角内三磷酸鸟苷环化水解酶1(GTP cyclohydrolase 1,GCH1)基因的表达变化与神经病理性疼痛产生的关系。方法:wistar大鼠64只被随机分为CCI组和Sham组,每组32只。对CCI组大鼠行右侧坐骨神经结扎;建立CCI模型,Sham组仅暴露坐骨神经不结扎,于术前1天及术后1、3、7、10、14、21、28天测定大鼠机械痛阈值,并分别在术后3、7、14、28天取腰4~6段脊髓,进行免疫组化和Western Blotting检测,观察脊髓背角GCH1表达的变化情况。结果:CCI组大鼠术后各时间点后肢机械痛阈值均显著低于Sham组(P0.05),术后第1天开始疼痛阈值便明显降低,直至术后第28天,均低于术前水平(P0.05)。免疫组化结果显示:CCI组大鼠术后脊髓背角神经元GCH1的表达呈先升高后降低的趋势;与Sham组相比,术后各时间点GCH1的表达均明显增高(P0.05)。Western Blotting检测与免疫组化结果基本一致,CCI组术后3天、7天、14天GCH1表达均高于Sham组(P0.05)。结论:周围神经损伤引起的痛觉过敏随脊髓背角内GCH1表达升高而增强,随其表达降低而减轻,可以认为GCH1可能参与了神经病理性疼痛的形成。
[Abstract]:Aim: to investigate the relationship between the expression of guanosine triphosphate cyclase 1 (GTP cyclohydrolase 1) gene and neuropathic pain after chronic constriction injuryCCI of sciatic nerve in rats. Methods 64 Wistar rats were randomly divided into CCI group and Sham group with 32 rats in each group. The right sciatic nerve was ligated in CCI group, the rats in CCI model Sham group were only exposed to no ligation of sciatic nerve, the mechanical pain threshold was measured 1 day before operation and 1 day after operation. The expression of GCH1 in the dorsal horn of spinal cord was observed by immunohistochemistry and Western blotting. Results the mechanical pain threshold of hind limbs of the rats in the 1: CCI group was significantly lower than that in the sham group at all time points, and the threshold value of the pain decreased significantly at the first day after operation, until the 28th day after the operation, it was lower than the preoperative level of P0.05. The results of immunohistochemistry showed that the expression of GCH1 in spinal dorsal horn neurons increased first and then decreased in the CCI group, compared with the Sham group, the expression of GCH1 in the spinal dorsal horn neurons increased at first and then decreased in the control group. The expression of GCH1 in CCI group was significantly higher than that in Sham group. The expression of GCH1 in CCI group was significantly higher than that in Sham group. The expression of GCH1 in CCI group was significantly higher than that in Sham group on the 3rd and 14th day after operation. The expression of GCH1 in CCI group was significantly higher than that in Sham group. Conclusion: the hyperalgesia induced by peripheral nerve injury increases with the increase of GCH1 expression in the dorsal horn of spinal cord and decreases with the decrease of GCH1 expression. It is suggested that GCH1 may be involved in the formation of neuropathic pain.
【作者单位】：济宁医学院附属医院脊柱外科;济宁医学院病理教研室;
【基金】：山东省教育厅资助课题(J11LF19)
【分类号】：R338

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