aFGF对新生大鼠海马星形胶质细胞存活与增殖的影响

发布时间：2018-06-20 06:37

本文选题：酸性成纤维细胞因子 + 庆大霉素　；参考：《现代预防医学》2013年18期

【摘要】：目的探讨酸性成纤维细胞因子(aFGF)对新生大鼠海马星形胶质细胞生长的影响及其对庆大霉素(GM)损伤海马星形胶质细胞的保护作用。方法取新生乳鼠,分离海马、剪切、胰蛋白酶消化,制成细胞悬液后接种。采用GTAP免疫组化染色鉴定。传3代细胞接种于96孔培养板或培养瓶:(1)培养24 h后加入一系列浓度的aFGF,再培养24 h后用MTT法检测细胞存活率;(2)GM组:2 g/L GM作用24 h;aFGF+GM组:加入一系列浓度aFGF 24h后再加2 g/L GM培养24 h,MTT法检测细胞存活率;观察细胞超微结构变化。结果星形胶质细胞在aFGF作用下有不同程度的增殖,并在一定范围内呈浓度依赖性(P㩳0.01);不同浓度的aFGF预作用24 h后,使GM致伤的星形胶质细胞存活率升高,并能减轻星形胶质细胞超微结构的破坏。结论 aFGF能促进大鼠海马星形胶质细胞的增殖及拮抗GM对星形胶质细胞的损伤作用。
[Abstract]:Objective to investigate the effects of acidic fibroblast factor (aFGF) on the growth of hippocampal astrocytes in neonatal rats and the protective effects of GMT on hippocampal astrocytes. Methods newborn rats were isolated from hippocampus, cut and digested by trypsin, then inoculated with cell suspension. GTAP immunohistochemical staining was used. Passage 3 cells were inoculated on 96 well culture plate or culture bottle 1) after 24 h culture, a series of concentration aFGFs were added. After 24 h culture, the cell survival rate was measured by MTT method. The cell survival rate was measured by MTT method. The cell survival rate was measured by MTT method in the 24 h GM-treated group with 1: 2 g / L GM: 24 h after a series of aFGF concentrations were added for 24 hours. The survival rate of the cells was measured by MTT assay after 24 h culture with 2 g / L GM. Ultrastructural changes were observed. Results astrocytes were proliferated in different degree under aFGF, and in a concentration dependent manner, the survival rate of GM injured astrocytes was increased after 24 h of aFGF pretreated with different concentrations of aFGF. And it can reduce the damage of astrocyte ultrastructure. Conclusion aFGF can promote the proliferation of hippocampal astrocytes and antagonize the injury of astrocytes induced by GM.
【作者单位】：广西医科大学护理学院;温州医学院生物与天然药物研究所;
【基金】：国家863计划课题(2004AA2Z3C60) 广西医疗卫生重点科研课题(2011046)
【分类号】：R329

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