氨基酸突变对蛋白质稳定性的影响预测及其在线工具

发布时间：2018-06-22 05:11

本文选题：点突变 + 蛋白质稳定性　；参考：《免疫学杂志》2013年11期

【摘要】：确定氨基酸突变对蛋白质稳定性的影响对于分子水平的疾病机理理解和新蛋白质设计是十分重要的。这项研究也有助于药物学上的为提高蛋白质药物的稳定性而对其进行的改造与重组以及免疫学上的疫苗设计。实验测定费时费力且代价昂贵。计算方法已用来预测突变的影响和阐明其潜在的生物学机制。本文阐述预测残基突变对蛋白质稳定性影响的两类方法:使用能量函数直接计算法和机器学习方法;介绍15个最新在线预测工具并说明面临的挑战与未来的方向。
[Abstract]:It is very important to determine the effect of amino acid mutation on protein stability for understanding the disease mechanism and designing new proteins at molecular level. The study also contributes to pharmacological modifications and recombination of protein drugs to improve their stability, as well as immunological vaccine design. Experimental measurements are time-consuming and costly. Computational methods have been used to predict the effects of mutations and to elucidate their potential biological mechanisms. In this paper, two kinds of methods for predicting the effect of residue mutation on protein stability are described: direct calculation of energy function and machine learning, and 15 new online prediction tools are introduced, and the challenges and future directions are described.
【作者单位】：新疆应用职业技术学院(原伊犁师范学院奎屯校区)文理系计算机教研室;新疆应用职业技术学院(原伊犁师范学院奎屯校区)教务处;
【基金】：新疆应用职业技术学院教改项目(JG2012001、JG2012008) 伊犁师范学院教改项目(JG2011057)
【分类号】：R341

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