急性重复低氧预适应小鼠海马组织DNA甲基转移酶表达的变化

发布时间：2018-06-24 22:02

本文选题：缺氧预适应 + 海马　；参考：《神经解剖学杂志》2013年06期

【摘要】：目的:检测小鼠脑海马组织中三种DNA甲基转移酶在急性重复缺氧条件下的变化,探讨缺氧预适应过程中DNA甲基转移酶的变化在低氧神经保护中的作用。方法:昆明小鼠随机分成正常对照组(常氧组,H0)、低氧1次组(H1)或低氧4次(H4)组,实验后取海马组织,应用Real-time PCR技术、Western bolt技术和DNA甲基转移酶活性实验对三种DNA甲基转移酶的变化进行研究。结果:DNA甲基转移酶1的mRNA和蛋白质水平在三组之间无明显变化;DNA甲基转移酶3A和3B mRNA和蛋白质水平在三组之间有变化,DNA甲基转移酶3A和3B的mRNA在H4组中表达较H0组降低,差异有统计学意义(P0.01),DNA甲基转移酶3A和3B的蛋白质变化与mRNA变化相似,H4组与H0组相比有所降低(P0.05);DNA甲基转移酶活性在H4组较H0组降低。结论:DNA甲基转移酶的变化可能参与缺氧预适应小鼠获得脑保护。
[Abstract]:Aim: to detect the changes of three kinds of DNA methyltransferases in mouse brain tissue under acute repeated hypoxia and to explore the role of DNA methyltransferases in hypoxic neuroprotection during hypoxia preconditioning. Methods: Kunming mice were randomly divided into normal control group (normoxic group), hypoxic group (H1) or hypoxic group (H4). The changes of three kinds of DNA-methyltransferases were studied by real-time bolt and DNA methyltransferase activity test. Results the mRNA and protein levels of DNA methyltransferase 1 did not change significantly among the three groups. The mRNA and protein levels of DNA methyltransferase 3A and 3B changed between the three groups. The mRNA expression of DNA methyltransferase 3A and 3B in H4 group was lower than that in H0 group. The protein changes of DNA methyltransferase 3A and 3B were similar to mRNA changes in H4 group and H0 group (P0.05). The activity of DNA methyltransferase in H4 group was lower than that in H0 group. Conclusion the changes of DNA methyltransferase may be involved in the brain protection of hypoxic preconditioning mice.
【作者单位】：包头医学院生物医学研究中心及基础医学部;首都医科大学低氧医学研究所;泰山医学院附属医院神经内科;
【基金】：国家自然科学基金(81060212;81160244) 内蒙古自然科学基金(2010BS1104) 中国博士后基金(20080430851) 内蒙古自治区高等学校科研(NJZY12221;NJZY12225) 内蒙古自治区高等学校青年科技英才支持计划资助(NJYT-13-A10) 教育部留学回国人员科研启动基金
【分类号】：R363

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