卡氏肺孢子菌肺炎生物被膜的体内观察及其β-防御素的分布特征研究

发布时间：2018-07-17 16:55

【摘要】：目的研究鼠卡氏肺孢子菌肺炎(Pneumocystis carinii,Pc)β-防御素的分布特征和观察卡氏肺孢子菌生物被膜。方法免疫抑制方法建立卡氏肺孢子菌肺炎动物模型,改良四胺银(GMS)染色和RT-PCR方法检测Pc病原体;银染色、过碘酸雪夫氏(PAS)染色和扫描电镜观察Pc的生物被膜;RT-PCR方法检测鼠肺、肾、脾、小肠、皮肤、肝脏、心以及血液8个组织的β1-防御素和β2-防御素的mRNA表达。结果免疫抑制诱导第5周PCR方法检测到Pc感染,第7周GMS染色观察到Pc包囊,第9周银染色、PAS染色和扫描电镜观察到明显的Pc生物被膜。RT-PCR方法在肺、肾、脾、小肠、皮肤、肝脏、心以及血液均检测到β1和β2防御素的mRNA表达,β1防御素在肾脏和皮肤表达最强,β2防御素在肺表达最强。结论 Pc在体内可以形成生物被膜,可以诱导宿主β防御素的产生;卡氏肺孢子菌肺炎肺部诱导的防御素以β2防御素为主。
[Abstract]:Objective to study the distribution of 尾 -defensin in Pneumocystis carinii Pc and observe the biofilm of Pneumocystis carinii. Methods the animal model of Pneumocystis carinii pneumonia was established by immunosuppressive method, the Pc pathogen was detected by modified tetraamine silver (GMS) staining and RT-PCR, and the lung was detected by silver staining, pas staining and scanning electron microscopy. MRNA expression of 尾 1-defensin and 尾 _ 2-defensin in kidney, spleen, small intestine, skin, liver, heart and blood. Results Pc infection was detected by PCR at the 5th week of immunosuppressive induction, Pc cysts were detected by GMS staining at week 7, and Pc biofilm was detected by silver staining and scanning electron microscopy at the 9th week. RT-PCR was observed in lung, kidney, spleen, small intestine and skin. The mRNA expressions of 尾 1 and 尾 2 defensins were detected in liver, heart and blood. The expression of 尾 1 defensin was the strongest in kidney and skin, and the strongest in lung. Conclusion\;
【作者单位】：重庆医科大学附属儿童医院临检中心;儿童发育疾病研究教育部重点实验室;儿科学重庆市重点实验室;重庆市儿童发育重大疾病诊治与预防国际科技合作基地;重庆医科大学病原生物教研室;
【基金】：国家自然科学青年基金(81000745) 重庆市卫生局医学科学技术研究项目(渝卫科教[2009]66号和(渝卫科教[2010]51号)~~
【分类号】：S852.61;R378

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