苏木乙酸乙酯提取物对AS模型大鼠LCATmRNA、ABCA1mRNA的影响
发布时间：2018-09-05 17:24
【摘要】：目的:通过观察动脉粥样硬化大鼠模型肝脏及腹主动脉病理形态学改变,观察对肝脏组织软磷脂胆固醇酰基转移酶(LCAT)、ATP结合盒转运体A1(ABCA1)表达的影响,探讨苏木乙酸乙酯提取物对动脉粥样硬化模型大鼠胆固醇逆转运的影响,以及苏木乙酸乙酯提取物防治动脉粥样硬化的作用机制,为临床应用苏木提供实验依据。方法:采用反复注射维生素D370万IU(kg/d)及高脂饮食喂养的方法,诱导SD大鼠建立动脉粥样硬化模型。成年大鼠40只,随机分为4组,分别为空白对照组、模型对照组、苏木乙酸乙酯提取物组、辛伐他汀组,每组10只,适应性喂养一周后,除空白对照组外余均腹腔注射维生素D370万IU(kg/d)持续3日,同时喂养高脂饮食12周,建立动脉粥样硬化模型。模型复制成功后各组连续给药28天,取材。采用HE染色法观察腹主动脉及肝脏病理形态学的变化,采用 RealTime PCR法观察肝脏 LCATmRNA及ABCA1mRNA的表达量的情况。结果:①肝脏病理形态学改变:空白对照组肝脏组织结构未见异常,肝细胞以中央静脉为中心呈放射状,肝细胞的结构清晰,无脂肪变性的细胞;模型组大鼠可见肝细胞肿胀,伴有大量多余的脂肪滴存在;苏木乙酸乙酯组大鼠肝脏可见轻度水肿,未见脂肪样空泡形成;辛伐他汀组大鼠肝细胞肿胀减轻,并伴有水样变性,偶可见极少量气球样变。②腹主动脉病理形态学改变:空白对照组腹主动脉血管壁内、中及外膜分界清楚;模型对照组血管内膜下可见平滑肌细胞排列紊乱、增生,同时可见极少量泡沫样细胞的出现,弹力纤维层发生变性;苏木乙酸乙酯组内皮细胞表面不光滑,少量平滑肌细胞排列紊乱、增生,中膜弹力纤维层排列不规则,外膜为纤薄的疏松结缔组织;辛伐他汀组内膜下可见有少量炎性细胞及脂质沉积、平滑肌细胞增生且排列紊乱,并有排列不规则的中膜弹力纤维层出现,外膜变薄。③RT-PCR结果显示:与空白组比较,各组肝脏组织中LCATmRNA表达显著降低,差异具有统计学意义(P0.05);与模型组比较,苏木乙酸乙酯提取物组及辛伐他汀组肝脏组织LCATmRNA的表达显著升高,差异具有统计学意义(P0.05);苏木乙酸乙酯提取物组与辛伐他汀组比较肝脏组织LCATmRNA表达量差异无统计学意义(P0.05)。④RT-PCR结果显示:与空白组比较,各组肝脏组织中ABCA1 mRNA表达显著降低,差异具有统计学意义(P0.05);与模型组比较,苏木乙酸乙酯提取物组及辛伐他汀组肝脏中ABCA1mRNA的表达量显著升高,差异具有统计学意义(P0.05);苏木乙酸乙酯提取物组与辛伐他汀组比较肝脏中ABCA1mRNA表达差异无统计学意义(P0.05)。结论:1、苏木乙酸乙酯提取物能够减轻动脉粥样硬化模型大鼠肝脏及腹主动脉病理形态学损伤。2、苏木乙酸乙酯提取物能够上调动脉粥样硬化模型大鼠LCATmRNA、ABCA1mRNA 水平的表达。
[Abstract]:Aim: to observe the effect of pathological morphology of liver and abdominal aorta on the expression of pellucidylcholinesterase (LCAT) -ATP-binding cassette transporter A1 (ABCA1) in rat model of atherosclerosis. To explore the effect of ethyl acetate extract of sapphire on cholesterol reverse transport in atherosclerotic rats and the mechanism of ethyl acetate extract in preventing and treating atherosclerosis in order to provide experimental basis for clinical application of sappan. Methods: the atherosclerosis model of SD rats was induced by repeated injection of vitamin D 3.7 million IU (kg/d) and high fat diet. Forty adult rats were randomly divided into 4 groups: blank control group, model control group, ethyl acetate extract group and simvastatin group. The model of atherosclerosis was established by intraperitoneal injection of vitamin D 3.7 million IU (kg/d) for 3 days with the exception of blank control group, and fed with a high-fat diet for 12 weeks. After the model was successfully duplicated, each group was given continuously for 28 days. The histopathological changes of abdominal aorta and liver were observed by HE staining and the expression of LCATmRNA and ABCA1mRNA in liver by RealTime PCR. Results the pathomorphologic changes of the liver in the control group were as follows: there was no abnormal structure in the liver in the blank control group, the liver cells were radially centered on the central vein, the structure of the liver cells was clear, and there were no fatty degeneration cells in the model group, while in the model group, the liver cells were swollen. A large number of excess fat droplets were present in the liver of rats in the ethyl sappan acetate group, mild edema was observed in the liver, and no adipose vacuoles were found in the liver. In simvastatin group, the swelling of liver cells was alleviated and accompanied by watery degeneration. In the blank control group, the boundary between the medial and outer membranes of the abdominal aorta was clear, and the smooth muscle cells were arranged disorderly and proliferated under the intima of the model control group. At the same time, the appearance of a very small number of foam like cells, elastic fiber layer denaturation, hematoxylacetate ethyl acetate group endothelial cells surface is not smooth, a small number of smooth muscle cells arranged disorder, proliferation, media elastic fiber layer arranged irregularly, In simvastatin group, there were a few inflammatory cells and lipid deposition, smooth muscle cells proliferated and disordered, and irregular arrangement of elastic fiber layer appeared. The results of RT-PCR showed that compared with the blank group, the expression of LCATmRNA in liver tissue of each group was significantly decreased (P0.05); compared with the model group, the expression of LCATmRNA in the liver tissue of each group was significantly lower than that of the control group (P0.05). The expression of LCATmRNA in liver tissue of ethyl acetate extract group and simvastatin group was significantly higher than that of simvastatin group. Compared with simvastatin group, there was no significant difference in LCATmRNA expression in liver tissue between ethyl acetate extract group and simvastatin group (P0.05). 4 RT-PCR results showed: compared with the blank group, the expression of ABCA1 mRNA in liver tissue of each group was significantly lower than that of simvastatin group. The difference was statistically significant (P0.05). Compared with the model group, the expression of ABCA1mRNA in the liver of the ethyl acetate extract group and simvastatin group was significantly higher than that in the model group. The difference was statistically significant (P0.05); there was no significant difference in the expression of ABCA1mRNA between the ethyl acetate extract group and simvastatin group (P0.05). Conclusion the ethyl acetate extract of hematoxylum sappan can attenuate the pathomorphological damage of liver and abdominal aorta of atherosclerotic rats by 0.2, and the extract of ethyl acetate can up-regulate the expression of LCATmRNA,ABCA1mRNA in atherosclerotic rats.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332
[Abstract]:Aim: to observe the effect of pathological morphology of liver and abdominal aorta on the expression of pellucidylcholinesterase (LCAT) -ATP-binding cassette transporter A1 (ABCA1) in rat model of atherosclerosis. To explore the effect of ethyl acetate extract of sapphire on cholesterol reverse transport in atherosclerotic rats and the mechanism of ethyl acetate extract in preventing and treating atherosclerosis in order to provide experimental basis for clinical application of sappan. Methods: the atherosclerosis model of SD rats was induced by repeated injection of vitamin D 3.7 million IU (kg/d) and high fat diet. Forty adult rats were randomly divided into 4 groups: blank control group, model control group, ethyl acetate extract group and simvastatin group. The model of atherosclerosis was established by intraperitoneal injection of vitamin D 3.7 million IU (kg/d) for 3 days with the exception of blank control group, and fed with a high-fat diet for 12 weeks. After the model was successfully duplicated, each group was given continuously for 28 days. The histopathological changes of abdominal aorta and liver were observed by HE staining and the expression of LCATmRNA and ABCA1mRNA in liver by RealTime PCR. Results the pathomorphologic changes of the liver in the control group were as follows: there was no abnormal structure in the liver in the blank control group, the liver cells were radially centered on the central vein, the structure of the liver cells was clear, and there were no fatty degeneration cells in the model group, while in the model group, the liver cells were swollen. A large number of excess fat droplets were present in the liver of rats in the ethyl sappan acetate group, mild edema was observed in the liver, and no adipose vacuoles were found in the liver. In simvastatin group, the swelling of liver cells was alleviated and accompanied by watery degeneration. In the blank control group, the boundary between the medial and outer membranes of the abdominal aorta was clear, and the smooth muscle cells were arranged disorderly and proliferated under the intima of the model control group. At the same time, the appearance of a very small number of foam like cells, elastic fiber layer denaturation, hematoxylacetate ethyl acetate group endothelial cells surface is not smooth, a small number of smooth muscle cells arranged disorder, proliferation, media elastic fiber layer arranged irregularly, In simvastatin group, there were a few inflammatory cells and lipid deposition, smooth muscle cells proliferated and disordered, and irregular arrangement of elastic fiber layer appeared. The results of RT-PCR showed that compared with the blank group, the expression of LCATmRNA in liver tissue of each group was significantly decreased (P0.05); compared with the model group, the expression of LCATmRNA in the liver tissue of each group was significantly lower than that of the control group (P0.05). The expression of LCATmRNA in liver tissue of ethyl acetate extract group and simvastatin group was significantly higher than that of simvastatin group. Compared with simvastatin group, there was no significant difference in LCATmRNA expression in liver tissue between ethyl acetate extract group and simvastatin group (P0.05). 4 RT-PCR results showed: compared with the blank group, the expression of ABCA1 mRNA in liver tissue of each group was significantly lower than that of simvastatin group. The difference was statistically significant (P0.05). Compared with the model group, the expression of ABCA1mRNA in the liver of the ethyl acetate extract group and simvastatin group was significantly higher than that in the model group. The difference was statistically significant (P0.05); there was no significant difference in the expression of ABCA1mRNA between the ethyl acetate extract group and simvastatin group (P0.05). Conclusion the ethyl acetate extract of hematoxylum sappan can attenuate the pathomorphological damage of liver and abdominal aorta of atherosclerotic rats by 0.2, and the extract of ethyl acetate can up-regulate the expression of LCATmRNA,ABCA1mRNA in atherosclerotic rats.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332
【参考文献】
相关期刊论文 前10条
1 刘孙夷;许嘉鸿;;非他汀类抗动脉粥样硬化药物治疗研究进展[J];同济大学学报(医学版);2016年04期
2 江小萍;曾凡鹏;刘首明;林小鸳;冯素莲;沈祖泓;;三七总皂苷对动脉粥样硬化患者血管炎症因子及颈动脉内膜中层厚度和斑块的影响[J];中国中医急症;2015年10期
3 杜文婷;刘萍;;丹参单体对动脉粥样硬化作用机制的研究概述[J];中西医结合心脑血管病杂志;2015年07期
4 Fulya Ilhan;Sevgi Tas Kalkanli;;Atherosclerosis and the role of immune cells[J];World Journal of Clinical Cases;2015年04期
5 孙龙飞;安冬青;;他汀类药物的多效性作用在抗动脉粥样硬化机制中的研究进展[J];中西医结合心脑血管病杂志;2015年04期
6 yぱ┟，
本文编号：2224936
本文链接：https://www.wllwen.com/yixuelunwen/jichuyixue/2224936.html
