生物钟基因Rev-erb-α缺失导致雌鼠生理周期延长

发布时间：2018-09-05 20:49

【摘要】：在哺乳动物中,生物节律与生殖生理和生殖内分泌密切相关,流行病学研究显示长期倒时差的女性表现出生殖力低下和不良妊娠的概率更高,但是人们对生物节律影响生殖的机制了解还很少。本研究旨在以时钟基因Rev-erb-α敲除型(Rev-erb-α-/-)雌性小鼠为模型初步探索生物节律对生殖的影响。将Rev-erb-α-/-和野生型(Rev-erb-α+/+)小鼠与C57BL/6J小鼠交配观察生殖力变化,用苏木精-伊红(HE)染色法观察卵巢组织变化,将Rev-erb-α+/+与Rev-erb-α-/-雌性小鼠分别与C57BL/6J雄性小鼠交配后比较见栓后1.5d统计的胚胎数量,用阴道涂片结合周期图和周期谱观察小鼠生理周期变化。结果显示,Rev-erb-α-/-雌性小鼠与C57BL/6J雄鼠交配是不生育的,并且Rev-erb-α-/-雌性小鼠生成的胚胎数量明显减少。Rev-erb-α-/-雌性小鼠卵巢存在各级卵泡,并且结构与野生型(Rev-erb-α+/+)雌性小鼠相比无明显差异。Rev-erb-α-/-雌性小鼠的生理周期较Rev-erb-α+/+小鼠周期长约3.22d,较Rev-erb-α+/+雌性小鼠延长63.5%。以上结果提示,生物钟基因Rev-erb-α缺失导致雌性小鼠生理周期明显延长,可能是影响雌性小鼠生育的原因之一。因此,生物节律的稳定对于生理周期具有重要作用。
[Abstract]:In mammals, biological rhythms are closely related to reproductive physiology and reproductive endocrine. Epidemiological studies have shown that women with chronic jet lag are more likely to have low fertility and poor pregnancy. But little is known about the mechanisms that biological rhythms affect reproduction. The aim of this study was to explore the effects of biological rhythm on reproduction in Rev-erb- 伪 -r- female mice with clock gene Rev-erb- 伪 knockout (Rev-erb- 伪 -r-). Rev-erb- 伪 -r- and wild type (Rev-erb- 伪 /) mice were mated with C57BL/6J mice to observe the change of fecundity, and the changes of ovarian tissue were observed by hematoxylin-eosin (HE) staining. After mating with C57BL/6J male mice, Rev-erb- 伪 / Rev-erb- 伪 -r-female mice were compared with the number of embryos counted 1.5 days after embolization. The physiological cycle changes of mice were observed by vaginal smear combined with cycle diagram and cycle spectrum. The results showed that the female mice copulated with C57BL/6J male mice were sterile, and the number of embryos produced in Rev-erb- 伪 -r-female mice was significantly reduced. Rev-erb- 伪 -r-female mouse ovaries had follicles of all levels. The physiological cycle of female mice was 3.22 days longer than that of Rev-erb- 伪 / female mice, and 63.5 days longer than that of Rev-erb- 伪 / female mice. These results suggest that the deletion of Rev-erb- 伪 of the biological clock gene may lead to the prolongation of physiological cycle in female mice, which may be one of the reasons that affect the fertility of female mice. Therefore, the stability of biological rhythm plays an important role in physiological cycle.
【作者单位】：温州医科大学浙江省模式生物技术与应用重点实验室;南京大学模式动物研究所模式动物疾病研究中心教育部重点实验室;苏州大学系统生物学中心;
【基金】：supported by the National Basic Research Development Program(973 Program)of China(No.2010CB945100)
【分类号】：R-332

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