间歇性低氧对大鼠海马区磷酸化JNK表达影响

发布时间：2018-10-05 06:25

【摘要】：目的探讨间歇性低氧对大鼠海马区磷酸化c-Jun氨基末端激酶(JNK)表达及学习记忆影响。方法120只雄性SD大鼠随机分成对照组及轻、重度低氧组,对照组暴露于空气中,低氧组分别暴露于不同低氧条件下(100、50 ml/L),暴露时间8 h/d,连续2、4、6、8周,Morris水迷宫检测学习记忆功能,光镜观察海马区神经细胞形态变化,免疫印迹和免疫组化法检测海马区磷酸化JNK表达。结果与对照组比较,随低氧时间延长,低氧组大鼠海马区脑组织结构损伤,磷酸化JNK表达增多;水迷宫检测动物逃避潜伏期延长、穿越原平台次数减少,与轻度低氧组比较,重度低氧组变化更为明显(P0.05);轻、重度低氧组暴露后6、8周大鼠海马区磷酸化JNK表达分别为(5.84±1.17)、(3.58±1.0)和(7.86±1.56)、(9.78±3.41),与轻度低氧组比较,重度低氧组大鼠海马区磷酸化JNK表达升高,差异有统计学意义(t=5.63,P0.05)。结论间歇性低氧可导致大鼠学习和记忆功能障碍,其机制可能与磷酸化JNK不同时间和强度差异表达有关。
[Abstract]:Objective to investigate the effects of intermittent hypoxia on the expression of phosphorylated c-Jun amino-terminal kinase (JNK) and learning and memory in rat hippocampus. Methods 120 male SD rats were randomly divided into control group and mild or severe hypoxic group. The control group was exposed to air, the hypoxic group was exposed to different hypoxic conditions (100 ~ 50 ml/L), the exposure time was 8 h / d, and the learning and memory function was measured by Morris water maze for 8 weeks. The morphological changes of hippocampal neurons were observed under light microscope, and the expression of phosphorylated JNK in hippocampus was detected by immunoblotting and immunohistochemistry. Results compared with the control group, with the prolongation of hypoxic time, the hippocampal tissue structure damage and phosphorylated JNK expression increased in the hypoxic group, and the escape latency prolonged and the number of crossing the original platform decreased in the water maze test group, compared with the mild hypoxic group. The expression of phosphorylated JNK was (5.84 卤1.17), (3.58 卤1.0) and (7.86 卤1.56), (9.78 卤3.41) in the hippocampus of the rats in the mild and severe hypoxic groups at 6 weeks after exposure. Compared with the mild hypoxia group, the expression of phosphorylated JNK in the hippocampus of the severe hypoxia group was higher than that of the mild hypoxia group. The difference was statistically significant (t = 5.63 P 0.05). Conclusion intermittent hypoxia may lead to learning and memory impairment in rats, and its mechanism may be related to the different expression of phosphorylated JNK at different time and intensity.
【作者单位】：河北联合大学康复医学院;河北联合大学附属医院呼吸内科;
【基金】：河北省科技厅资助项目(09276103D-11)
【分类号】：R363

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