酒精性痴呆大鼠模型的建立方法及评价

发布时间：2018-10-09 14:06

【摘要】：目的通过比较不同酒精作用途径、剂量和时间对学习记忆功能的影响,寻求酒精性痴呆(alcoholassociated dementia,AAD)动物模型的最佳建立方法。方法以SD大鼠为实验对象,选择不同剂量酒精(20%,4～12mL/kg)连续腹腔注射和灌胃,持续周期为14d和28d,并以Morris水迷宫行为学检测、大鼠海马组织形态学变化和神经细胞凋亡检测作为AAD动物模型的评价标准。结果与生理盐水灌胃组〔(5.68±1.30)s〕相比,大鼠在给予低剂量酒精(4mL/kg)灌胃14d后,其逃避潜伏期时间显著延长〔(24.70±5.43)s,P0.05〕,提示其学习记忆功能受损害;而高剂量(12mL/kg)酒精灌胃组其逃避潜伏期虽有延长趋势〔(9.55±2.30)s〕,但无统计学差异。与生理盐水腹腔注射组〔(5.31±0.85)s〕相比,酒精腹腔注射组大鼠的逃避潜伏期时间亦延长〔(12.49±2.82)s,P0.05〕。低剂量酒精灌胃可诱导大鼠海马形态结构改变以及促使神经细胞显著凋亡,但高剂量酒精灌胃和酒精腹腔注射组改变不明显。结论酒精灌胃可诱导大鼠学习记忆功能明显损害,是ADD建模的适宜方法,但酒精剂量不宜过高。
[Abstract]:Objective to explore the best method for establishing alcoholic dementia (alcoholassociated dementia,AAD) animal model by comparing the effects of different alcohol pathways, doses and time on learning and memory function. Methods SD rats were selected for continuous intraperitoneal injection and gavage with different doses of alcohol (204ml / kg) for 14 days and 28 days. The behavior of Morris water labyrinth was measured. The morphologic changes of hippocampus and neuronal apoptosis were used as the evaluation criteria of AAD animal model. Results compared with the saline group (5.68 卤1.30) s), the escape latency of rats was significantly prolonged (24.70 卤5.43) (P 0.05) for 14 days after the administration of low-dose alcohol (4mL/kg), indicating that the learning and memory function of rats was impaired. In high dose (12mL/kg) group, the escape latency was prolonged (9.55 卤2.30) s), but there was no statistical difference. Compared with saline intraperitoneal injection group (5.31 卤0.85) s), the escape latency of rats in ethanol intraperitoneal injection group was also prolonged (12.49 卤2.82) (P0.05). The changes of hippocampal morphology and neuronal apoptosis were induced by low-dose alcohol administration, but the changes were not obvious in high-dose alcohol administration group and ethanol intraperitoneal injection group. Conclusion Alcohol administration can induce significant impairment of learning and memory function in rats. It is a suitable method for ADD modeling, but alcohol dose should not be too high.
【作者单位】：江西省人民医院临床医学研究所
【基金】：国家自然科学基金课题(No.81060111)
【分类号】：R749.6;R-332

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