酸性成纤维细胞生长因子对人脐带血内皮祖细胞凋亡的影响

发布时间：2018-10-26 09:44

【摘要】：目的探讨酸性成纤维细胞生长因子(aFGF)对人脐带血中内皮祖细胞凋亡的影响及机制。方法选择健康产妇脐带血,密度梯度离心法获取脐带血单个核细胞,培养7天后,收集贴壁细胞并加入不同浓度(2、5、10μg/L)aFGF干预24 h,流式细胞仪检测aFGF对细胞凋亡的影响,逆转录-聚合酶链反应检测Bcl-2 mRNA表达,Western Blot检测Bcl-2蛋白表达。同时对作用效果最为显著的组(5μg/L aFGF组)分别培养6、12、24、48 h,分别观察内皮祖细胞数量及凋亡状况,从而对其时效关系进行观察。结果与对照组比较,不同浓度的aFGF可以显著抑制内皮祖细胞凋亡(P0.05);5μg/L aFGF作用24 h时对细胞凋亡抑制的影响最为显著(P0.05)。Bcl-2mRNA和蛋白的表达显著高于对照组。结论 aFGF能抑制人脐带血内皮祖细胞的凋亡,并通过上调凋亡抑制基因Bcl-2而发挥抑制细胞凋亡的作用。
[Abstract]:Objective to investigate the effect and mechanism of acidic fibroblast growth factor (aFGF) on endothelial progenitor cell apoptosis in human umbilical cord blood. Methods umbilical cord blood mononuclear cells were obtained by density gradient centrifugation. After 7 days of culture, adherent cells were collected and treated with 10 渭 g / L aFGF for 24 h. The effect of aFGF on apoptosis was detected by flow cytometry, Bcl-2 mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Bcl-2 protein expression was detected by, Western Blot. At the same time, the most effective group (5 渭 g / L aFGF) was cultured for 48 hours, and the number and apoptosis of endothelial progenitor cells were observed, and the time-dependent relationship was observed. Results compared with the control group, different concentrations of aFGF could significantly inhibit the apoptosis of endothelial progenitor cells (P0.05). 5 渭 g / L aFGF had the most significant effect on the inhibition of apoptosis at 24 h (P0.05). The expression of Bcl-2mRNA and protein was significantly higher than that of the control group. Conclusion aFGF can inhibit the apoptosis of human umbilical cord blood endothelial progenitor cells and inhibit the apoptosis by up-regulating the apoptosis suppressor gene Bcl-2.
【作者单位】：温州医学院附属第三医院普通外科;
【分类号】：R329

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