少突胶质细胞前体细胞在双环己酮草酰二腙诱导的脱髓鞘模型中的变化特点

发布时间：2018-10-26 20:23

【摘要】：目的观察神经胶质细胞在双环己酮草酰二腙(cuprizone,CPZ)诱导的脱髓鞘模型中的变化特点,探讨其与髓鞘再生的内在联系。方法选8周龄C57BL/6雄性小鼠,分为正常组、急性脱髓鞘组及髓鞘再生组。正常组每天饲养正常鼠粮;模型组小鼠饲养含有0.2%CPZ的混合鼠粮,连续饲养6周;髓鞘再生组在连续喂养6周CPZ鼠粮后,换用正常鼠粮2周。实验过程中通过卢卡斯快蓝染色及透射电镜技术判断脑组织胼胝体区髓鞘脱失及恢复程度,通过免疫组化及免疫荧光技术检测脑内NG2,Olig2和GFAP的表达来评估少突胶质细胞前体细胞及星形胶质细胞的变化特点。结果与正常组相比,急性脱髓鞘组胼胝体髓鞘脱失明显,Olig2、GFAP表达明显升高(P0.05),侧脑室旁外侧隔核(LSD)NG2升高水平较胼胝体区(CC)更为明显(P0.05,P0.01);髓鞘再生组髓鞘有所修复,Olig2、GFAP表达仍高于正常组,但较急性脱髓鞘组有一定程度降低(P0.05)。结论髓鞘损伤期少突胶质细胞前体细胞不能有效迁移至受损部位,修复期Olig2表达降低,星形胶质细胞持续高水平表达可能是髓鞘修复障碍的影响因素。
[Abstract]:Aim to observe the changes of glial cells in demyelinating model induced by dicyclohexanone oxalyl dihydrazone (cuprizone,CPZ) and to explore the relationship between glial cells and myelin regeneration. Methods 8 week old male C57BL/6 mice were divided into normal group, acute demyelinating group and myelin regeneration group. The normal diet was fed to normal mice daily in normal group, the mixed diet containing 0.2%CPZ was fed to mice in model group for 6 weeks, and the myelin regeneration group was replaced with normal rat grain for 2 weeks after 6 weeks of continuous feeding. The degree of demyelination and recovery of corpus callosum was determined by Lucas fast blue staining and transmission electron microscopy. NG2, in brain was detected by immunohistochemistry and immunofluorescence technique. The expression of Olig2 and GFAP was used to evaluate the characteristics of oligodendrocyte progenitor cells and astrocytes. Results compared with the normal group, the corpus callosum demyelination and the expression of Olig2,GFAP were significantly increased in the acute demyelinating group (P0.05). The increased level of (LSD) NG2 in the lateral paraventricular septal nucleus was significantly higher than that in the corpus callosum area (P0.05). P0.01); The myelin sheath was repaired and the expression of Olig2,GFAP was still higher in the regenerated myelin group than that in the acute demyelinating group (P0.05). Conclusion the oligodendrocyte precursor cells could not migrate to the damaged site effectively during myelin sheath injury, but the expression of Olig2 decreased during the repair period. The sustained high expression of astrocytes may be the influencing factor of myelin repair disorder.
【作者单位】：第三军医大学西南医院神经内科;第三军医大学西南医院康复科;
【分类号】：R-332

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