大鼠骨髓基质干细胞体外诱导分化为神经元样细胞相关基因研究

发布时间：2018-11-01 11:16

【摘要】：目的探索大鼠骨髓基质干细胞(bone marrow stromal cells,BMSCs)体外定向诱导分化为神经元样细胞所涉及的相关基因,并探讨这些基因在BMSCs诱导分化为神经元样细胞时所起的作用。方法体外培养BMSCs至第4代,以碱性成纤维细胞生长因子和表皮生长因子诱导7d,于诱导前和诱导后第7天抽提和纯化RNA,采用Affymetrix U133 plus 2.0基因表达谱芯片及技术对诱导前后的所有基因进行比较,找出其中的差异表达基因,并对其功能进行研究。结果通过对诱导前后的所有基因进行比较,总共找到97条差异基因,其中上调基因62条,下调基因35条。明确与神经元发育密切相关的基因有24条,其中上调18条,下调6条。结论 BMSCs体外诱导分化为神经元样细胞的相关基因表达谱中,有一些与神经元分化密切相关的基因存在差异表达,深入研究这些基因可能为基因工程改造BMSCs奠定基础。
[Abstract]:Objective to explore the related genes involved in directional differentiation of rat bone marrow stromal cells (bone marrow stromal cells,BMSCs) into neuron-like cells in vitro and to explore the role of these genes in differentiation into neuron-like cells induced by BMSCs. Methods BMSCs was cultured in vitro for 7 days after induction with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). RNA, was extracted and purified before and 7 days after induction. Affymetrix U133 plus 2.0 gene expression microarray and technique were used to compare all the genes before and after induction, to find out the differentially expressed genes, and to study their functions. Results by comparing all the genes before and after induction, 97 differentially expressed genes were found, among which 62 genes were up-regulated and 35 genes were down-regulated. There were 24 genes related to neuron development, including 18 up-regulated genes and 6 down-regulated genes. Conclusion there are some differentially expressed genes related to neuronal differentiation in the gene expression profiles of neuron-like cells induced by BMSCs in vitro. Further study of these genes may lay a foundation for BMSCs gene engineering.
【作者单位】：苏州市吴中人民医院神经外科;苏州市中医医院神经外科;苏州大学附属第二医院神经外科;
【基金】：江苏省自然科学基金资助项目(BK2004043)
【分类号】：R329

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