大鼠骨髓来源的内皮祖细胞培养方法的优化

发布时间：2018-11-01 14:20

【摘要】：目的探索提取和培养内皮祖细胞的改良方法。方法采用密度离心法从大鼠骨髓中分离单个核细胞,接种于预包被纤维连接蛋白的培养皿中,使用不同的培养条件进行培养,利用差速贴壁法和差速消化法纯化经诱导培养得到的内皮祖细胞。对得到的内皮祖细胞进行体外成血管功能的鉴定后,比较不同培养条件下得到的内皮祖细胞的数量。结果使用M199培养基,添加10%FBS,10 ng/mLVEGF,5 ng/mL bFGF,2 ng/mL CSF的培养条件最适合内皮祖细胞的生长。结论经过提取和培养方法的改良,可以从大鼠骨髓中提取得到数量较多的成集落生长的内皮祖细胞,培养得到的内皮祖细胞在体外试验中表现出新生血管的能力。
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【作者单位】：哈尔滨医科大学附属第二医院血管外科;哈尔滨医科大学附属第四医院血管外科;
【基金】：黑龙江省自然科学基金资助项目(D2007-11) 哈尔滨市科委青年科技创新人才基金(2007RFQXS084)
【分类号】：R329

【参考文献】