β-淀粉样蛋白对大鼠海马星形胶质细胞N-甲基-D-天冬氨酸受体亚单位表达的影响

发布时间：2018-11-03 20:37

【摘要】：目的探讨Wistar大鼠海马星形胶质细胞N-甲基-D-天冬氨酸受体(NMDAR)亚单位在β-淀粉样蛋白(Aβ)25～35毒性作用下的表达变化特点。方法大鼠海马原代培养细胞,加Aβ25～35(10μmol/L)分别作用1h、24h后,应用免疫荧光方法检测对照组和加药组中NR1、NR2A和NR2B的表达情况(n=10)。结果对照组海马星形胶质细胞表达NR1、NR2A和NR2B,阳性点状颗粒主要分布在细胞胞体和突起上,在胞体部位分布密集,在细胞突起则散在分布。经Aβ25～35作用后,三者表达均显著增强(P0.05)。Aβ1h组和24h组相比,NR1无显著变化,NR2A、NR2B的表达随作用时间增加显著增强(P0.05)。结论正常状态下海马星形胶质细胞可表达NMDAR亚单位(NR1、NR2A和NR2B);Aβ25～35作用会引起NMDAR各亚单位表达显著增强,NR1的表达变化与NR2A和NR2B的变化表现出不一致性。
[Abstract]:Objective to investigate the expression of N-methyl-D-aspartate receptor (NMDAR) subunit in hippocampal astrocytes of Wistar rats under the toxicity of 尾 -amyloid protein (A 尾) 2535.AIM: to investigate the expression of N-methyl-D-aspartate receptor (NMDAR) subunit in hippocampal astrocytes of Wistar rats. Methods Rat hippocampal primary cultured cells were treated with A 尾 25N 35 (10 渭 mol/L) for 1 h or 24 h, respectively. The expression of NR1,NR2A and NR2B in the control group and the drug added group were detected by immunofluorescence assay (nm10). Results in the control group, the expression of NR1,NR2A and NR2B, in astrocytes was mainly distributed on the cell body and process, densely distributed in the cell body, and scattered in the cell process. After treatment with A 尾 25, the expression of NR1 was significantly increased (P0.05). A 尾 1 h group and 24 h group, no significant change in NR1, NR2A,NR2B expression increased significantly with the time of action (P0.05). Conclusion under normal condition, the expression of NMDAR subunits in hippocampal astrocytes (NR1,NR2A and NR2B); A 尾 2535) can significantly increase the expression of NMDAR subunits, and the changes of NR1 expression are inconsistent with NR2A and NR2B changes.
【作者单位】：首都医科大学基础医学院人体解剖学教研室;
【基金】：新世纪优秀人才支持计划资助项目(NCET-10-0015) 国家自然科学基金资助项目(81071013) 北京市自然科学基金资助项目(7102011) 北京市教委科技发展计划资助项目(SQKM201210025003) 北京市脑重大疾病重点实验室资助项目
【分类号】：R363

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