日本血吸虫感染进程中小鼠脾中专职性和非专职性抗原提呈细胞的动态变化

发布时间：2018-11-07 11:35

【摘要】：目的观察日本血吸虫感染过程中Th2型免疫应答出现前后不同树突状细胞(DC)亚群、巨噬细胞、肥大细胞、嗜酸粒细胞和嗜碱粒细胞比例在小鼠脾中的变化。方法20条日本血吸虫尾蚴经腹部皮肤感染C57BL/6小鼠建立实验模型。在感染前和感染后2、4和6周处死小鼠,无菌分离小鼠脾,制备单细胞悬液。流式染色方法检测肥大细胞、嗜酸粒细胞和嗜碱粒细胞,并对DC和巨噬细胞进行分群,同时对其在脾非T非B细胞中的比例进行统计学分析。结果感染后4周,CD11c+CD8+DC在B220-DC中的比例从感染前的7.4%迅速增至17.1%;CD11c+CD4+DC从感染前的7.9%增至12.0%。从感染前到感染后6周,CD11c+CD4-CD8-DC在B220-DC中的比例基本保持恒定,维持在70%左右;浆细胞样DC(B220+CD11c+)在脾非T非B细胞中的比例呈现逐渐下降的趋势。感染后4周非T非B细胞中两群巨噬细胞(F4/80+CD11bint,F4/80+CD11bhigh)的比例(2.7%,8.6%)均明显低于感染前(15.4%,13.7%)。F4/80+细胞中高表达CD11b的巨噬细胞的比例从感染前47.1%增至感染后4周的75.5%。随着感染时间的延长,嗜酸粒细胞在小鼠脾中的比例逐渐增加,而嗜碱粒细胞和肥大细胞在脾中非T非B细胞中所占的比例却逐渐下降。结论 Th2应答出现前CD11c+CD8+DC和CD11c+CD4+DC亚群比例增高,而Th2应答出现后嗜酸粒细胞的比例显著增加。
[Abstract]:Objective to observe the changes of (DC) subsets, macrophages, mast cells, eosinophils and basophil ratios of different dendritic cells in mice before and after the appearance of Th2 type immune response during Schistosoma japonicum infection. Methods C57BL/6 mice were infected with 20 cercariae of Schistosoma japonicum through abdominal skin. Mice were killed before infection and 2 weeks and 6 weeks after infection. The spleen of mice was isolated aseptically and single cell suspension was prepared. Mast cells, eosinophil and basophil were detected by flow cytometry. DC and macrophages were divided into two groups, and the proportion of mast cells, eosinophil and eosinophil in spleen non-T non-B cells was analyzed statistically. Results four weeks after infection, the proportion of CD11c CD8 DC in B220-DC increased rapidly from 7.4% to 17.1%, from 7.9% to 12.0%. From pre-infection to 6 weeks after infection, the proportion of CD11c CD4-CD8-DC in B220-DC remained stable, about 70%, and the proportion of plasma cell-like DC (B220 CD11c) in non-T non-B cells of spleen decreased gradually. Four weeks after infection, the ratio of two groups of macrophages (F4 / 80 CD11bint,F4/80 CD11bhigh) in non-T non-B cells (2.7%) was significantly lower than that before infection (15.4%). The percentage of macrophages with high expression of CD11b in F4 / 80 cells increased from 47.1% before infection to 75.5% at 4 weeks after infection. The proportion of eosinophil in mouse spleen increased gradually with the extension of infection time, while the proportion of basophil and mast cell in spleen non-T non-B cells decreased gradually. Conclusion the proportion of CD11c CD8 DC and CD11c CD4 DC subsets was increased before Th2 response and the percentage of eosinophils increased significantly after Th2 response.
【作者单位】：同济大学医学院免疫学教研室;
【基金】：国家自然科学基金(No.81072382)~~
【分类号】：R392

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