人脑和大鼠新皮层FS神经元的电生理学特征
发布时间：2018-11-19 19:07
【摘要】：目的:研究人脑与大鼠新皮层FS神经元的电生理学特征及异同点。方法:应用手术切除非病理皮层组织制备和培养人脑脑片,利用全细胞膜片钳记录FS神经元的内在电生理特征,比较人脑与大鼠新皮层FS神经元电生理学差异。结果:(1)根据神经元在阈强度水平最初发放的动作电位放电模式,可将人脑FS神经元分为三种类型:典型性FS神经元(c-FS),爆发性FS神经元(b-FS)及延迟性FS神经元(d-FS)。这三种亚型类似于大鼠大脑皮层FS神经元的电生理学分类亚型。(2)人脑FS神经元的动作电位阈值、后超极化幅度及输入电流/输出频率斜率(I/O slope)均较大鼠增高,而静息电位、动作电位半宽及阈强度均较大鼠降低。人脑皮层神经元动作电位半宽和动作电位后超极化幅度(人:-24.61±2.45)较大鼠神经元的差异具有显著性意义(P0.05)。(3)当输入相同电流时人脑皮层神经元动作电位的输出频率大于大鼠神经元,当输入电流为75 p A时具有显著性差异(P0.05)。结论:(1)人脑FS神经元的电生理学分类与大鼠相似;(2)人脑FS神经元与大鼠存在电生理学差异;(3)人脑FS神经元在输入电流接近75 p A时动作电位频率与大鼠存在差异。
[Abstract]:Aim: to study the electrophysiological characteristics and similarities and differences of FS neurons in human brain and rat neocortex. Methods: brain slices were prepared and cultured by surgical excision of non-pathological cortical tissue. The intrinsic electrophysiological characteristics of FS neurons were recorded by whole-cell patch clamp. The electrophysiological differences of FS neurons between human brain and rat neocortex were compared. Results: (1) according to the pattern of action potential discharge initially released by neurons at threshold intensity level, human FS neurons can be classified into three types: typical FS neurons (c-FS). Explosive FS neurons (b-FS) and delayed FS neurons (d-FS). These three subtypes are similar to the electrophysiological subtypes of FS neurons in rat cerebral cortex. (2) the threshold of action potential of human brain FS neurons, the amplitude of posthyperpolarization and the I / O slope increase. The resting potential, half width of action potential and threshold intensity decreased significantly. There were significant differences in the half width of action potential and the amplitude of hyperpolarization after action potential (human: -24.61 卤2.45) in cerebral cortical neurons (P0.05). (3). The output frequency of action potential of laminar neurons was higher than that of rat neurons. There was significant difference when the input current was 75 Pa (P0.05). Conclusion: (1) the electrophysiological classification of FS neurons in human brain is similar to that in rats, (2) there are differences in electrophysiology between human brain FS neurons and rats. (3) the frequency of action potential of human brain FS neurons was different from that of rats when the input current was close to 75 Pa.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R338
本文编号：2343174
[Abstract]:Aim: to study the electrophysiological characteristics and similarities and differences of FS neurons in human brain and rat neocortex. Methods: brain slices were prepared and cultured by surgical excision of non-pathological cortical tissue. The intrinsic electrophysiological characteristics of FS neurons were recorded by whole-cell patch clamp. The electrophysiological differences of FS neurons between human brain and rat neocortex were compared. Results: (1) according to the pattern of action potential discharge initially released by neurons at threshold intensity level, human FS neurons can be classified into three types: typical FS neurons (c-FS). Explosive FS neurons (b-FS) and delayed FS neurons (d-FS). These three subtypes are similar to the electrophysiological subtypes of FS neurons in rat cerebral cortex. (2) the threshold of action potential of human brain FS neurons, the amplitude of posthyperpolarization and the I / O slope increase. The resting potential, half width of action potential and threshold intensity decreased significantly. There were significant differences in the half width of action potential and the amplitude of hyperpolarization after action potential (human: -24.61 卤2.45) in cerebral cortical neurons (P0.05). (3). The output frequency of action potential of laminar neurons was higher than that of rat neurons. There was significant difference when the input current was 75 Pa (P0.05). Conclusion: (1) the electrophysiological classification of FS neurons in human brain is similar to that in rats, (2) there are differences in electrophysiology between human brain FS neurons and rats. (3) the frequency of action potential of human brain FS neurons was different from that of rats when the input current was close to 75 Pa.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R338
【参考文献】
中国硕士学位论文全文数据库 前1条
1 王玉晶;神经科学研究中人类脑片培养技术的建立和评价[D];吉林大学;2015年
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