腺嘌呤核苷受体介导肿瘤坏死因子-α开放血肿瘤屏障的作用及其机制

发布时间：2018-12-19 10:29

【摘要】：目的明确肿瘤坏死因子-α(TNF-α)开放血肿瘤屏障的作用及其可能机制。方法构建体外血肿瘤屏障模型,给予TNF-α后不同时间点,免疫组织化学法和Western blot法动态检测胶质瘤细胞的热休克蛋白70(heat shock protein70,HSP70)和腺嘌呤核苷受体(adenosine A1receptor,A1R)的表达水平;荧光分析法检测体外血肿瘤屏障模型的通透性改变。结果给予TNF-α后,胶质瘤细胞内HSP70表达增加,于30 min时含量最多。C6细胞内A1R的表达水平也在给予TNF-α后明显增加,并于120 min达高峰后逐渐减少。其血肿瘤屏障的通透性亦于给予TNF-α后120 min达最大后减少。结论经TNF-α作用后的胶质瘤细胞HSP70表达增加,增加的HSP70可能是通过某种内在机制提高A1R的表达水平,进而增加了血肿瘤屏障的通透性。
[Abstract]:Objective to investigate the role of tumor necrosis factor- 伪 (TNF- 伪) in opening the blood-tumor barrier and its possible mechanism. Methods the blood tumor barrier model was established in vitro. At different time points after TNF- 伪 administration, the heat shock protein 70 (heat shock protein70,HSP70 (HSP70) and adenosine A1 receptor (adenosine A1 receptor) of glioma cells were detected by immunohistochemistry and Western blot assay. A1R) expression level; The permeability of blood tumor barrier model in vitro was detected by fluorescence assay. Results after TNF- 伪 administration, the expression of HSP70 increased in glioma cells, and reached the highest level at 30 min. The expression of A1R in C6 cells also increased significantly after TNF- 伪 administration, and gradually decreased after the peak of 120 min. The permeability of blood tumor barrier was also decreased after TNF- 伪 was given to 120 min. Conclusion the expression of HSP70 in glioma cells treated with TNF- 伪 is increased, and the increased HSP70 may increase the expression of A1R through some internal mechanism, thus increasing the permeability of blood tumor barrier.
【作者单位】：河北联合大学基础医学院;沈阳药科大学生命科学与生物制药学院生理学教研室;河北联合大学中医学院;中国医科大学基础医学院神经生物学教研室;
【基金】：国家自然科学基金资助项目(No 81101912) 河北省卫生厅科学研究基金项目(No 20120144) 国家教育部新教师基金项目(No 20102134120007) 辽宁省博士启动基金项目(No 20101109)
【分类号】：R341

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