基于数字化定量的高尿酸血症鹌鹑模型“四诊”证候研究

发布时间：2018-12-19 11:24

【摘要】：目的客观量化鹌鹑尿酸(UA)、粪尿及舌象等指标,探讨高尿酸血症鹌鹑模型的中医证候分型,为鹌鹑证候模型评价研究提供参考。方法采用高嘌呤饮食诱导鹌鹑高尿酸血症模型,动态观察鹌鹑尿酸水平、粪尿性状及舌象并进行数字化定量分析。运用健脾利湿中药菊苣进行反证,从"四诊"及以药测证两个角度探讨高尿酸血症鹌鹑中医证候类型。结果问诊、闻诊及望诊显示,实验第17、30天模型组鹌鹑粪尿湿重有升高趋势,粪尿较溏稀气味较大,正常组鹌鹑舌色以红色调为主,模型组鹌鹑舌色变淡呈淡白舌;第22、32天舌象红色度(red intensity,r)及红色面积比例显著下降;与正常组比,第14、21天血清尿酸水平显著升高(P0.05)。与模型组比,菊苣组粪尿湿重、干重显著升高(P0.05);r及红色面积比例显著上升(P0.05);第21天菊苣组尿酸水平显著降低(P0.05)。结论采用高嘌呤饮食诱导法,可成功诱导鹌鹑高尿酸血症。量化"四诊"指标及以药测证研究提示:鹌鹑模型部分病理改变与饮食不节导致的"脾虚痰湿"证候有一定的相似之处。
[Abstract]:Objective to study the TCM syndromes of quail model with hyperuricemia by objectively quantifying the indexes of (UA), fecal urine and tongue, and to provide reference for the evaluation of quail syndrome model. Methods the hyperuricemia model of quail was induced by hyperpurine diet. The syndrome types of hyperuricemia quail were discussed from the two angles of "four diagnosis" and "drug test" by using invigorating spleen and dampness traditional Chinese medicine chicory. Results the results showed that the wet weight of quail feces and urine in the model group increased on the 17th day of the experiment, the smell of feces and urine was larger than that of loose stool, the color of the tongue of the normal group was mainly red, and the color of the model group was light and white. On the 22nd day, the red intensity,r and the ratio of red area decreased significantly, and the serum uric acid level increased significantly at day 1421 compared with the normal group (P0.05). Compared with the model group, the feces and urine wet weight and dry weight of chicory group increased significantly (P0.05); r and red area ratio significantly increased (P0.05); on the 21st day, the uric acid level of chicory group decreased significantly (P0.05). Conclusion hyperuricemia can be induced successfully by hyperpurine diet. The study of quantifying the index of "four diagnoses" and examining the syndrome by medicine indicated that there were certain similarities between the pathological changes of quail model and the syndrome of "spleen deficiency and phlegm dampness" caused by diet.
【作者单位】：北京中医药大学;
【基金】：国家自然科学基金资助项目(No.81073068,81274153) 北京中医药大学研究生自主立项课题(No.2011-JYBZZ-XS067) 北京中医药大学科研创新团队项目(No.2011-CXTD-14)
【分类号】：R-332

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