玻璃化冷冻对人卵巢组织形态结构及组织增殖活性的影响

发布时间：2018-12-21 09:04

【摘要】：目的:探讨玻璃化冷冻对人类卵巢组织卵泡形态、超微结构和组织增殖活性的影响。方法:采用12例人卵巢组织切成薄片随机分配到新鲜卵巢组(A组)和玻璃化冷冻组(B组),两组行组织学光镜和透射电镜检查卵泡形态及结构变化,免疫组化测定卵巢组织中增殖细胞核抗原(PCNA)的表达情况。结果:A、B两组中原始卵泡和初级卵泡分布比例分别为86.4%、13.6%和84.5%%、15.5%,两组比较差异无统计学意义(P0.05)。光镜下A、B两组中形态正常的原始卵泡分别为75.3%和69.3%,两组比较差异亦无统计学意义(P0.05);但B组冻融后初级卵泡形态异常的比例(56%)高于A组(28.2%),两组比较差异有统计学意义(P0.05);A、B组中形态正常的原始卵泡超微结构无明显改变,B组中初级卵泡超微结构存在一定程度的改变。A、B两组卵巢中PCNA阳性表达比较差异无统计学意义(P0.05),PCNA阳性表达主要见于原始和初级卵泡的卵母细胞、颗粒细胞和卵巢组织间质细胞。结论:玻璃化冷冻是一种较适宜保存人类卵巢组织的方法。
[Abstract]:Aim: to investigate the effects of vitrification on the morphology, ultrastructure and proliferative activity of human ovarian tissue. Methods: 12 cases of human ovarian tissue were randomly divided into fresh ovary group (group A) and vitrification group (group B). The morphological and structural changes of follicles were examined by histological microscopy and transmission electron microscope. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. Results: the distribution ratio of primordial follicles and primary follicles was 86.6% and 84.5% in group A and B, respectively. There was no significant difference between the two groups (P0.05). Under light microscope, the normal primordial follicles were 75.3% and 69.3% in group A and B, respectively, and there was no significant difference between the two groups (P0.05). But the rate of abnormal primary follicle morphology in group B (56%) was higher than that in group A (28.2%), the difference was statistically significant (P0.05). The ultrastructure of primordial follicles in group A and B had no significant changes, but the ultrastructure of primary follicles in group B. there was no significant difference in the expression of PCNA between the two groups (P0.05). The positive expression of PCNA was mainly found in oocytes, granulosa cells and stromal cells of primary and primary follicles. Conclusion: vitrification is a suitable method for the preservation of human ovarian tissue.
【作者单位】：浙江大学医学院附属妇产科医院;
【基金】：浙江省教育厅资助项目(Y200804303)
【分类号】：R321.1

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